Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NAG20 / UBAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130020-100
Catalog Number: LS-G130020
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UBAP1 (NM_001171201), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- UAP | UBAP-1 | Ubiquitin-associated protein 1 | Ubiquitin associated protein | NAG20 | UBAP | UBAP1 | Ubiquitin associated protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_016525
- SwissProt
- Q9NZ09
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGAVRGRRR EVGLQHGGCG TGGGYGDGLA RSGQSWRWWR SLSLGAVGSS AGTEPGRPAG61ASTFRLLRRR QQRHSGTFSY LDDVPFKTGD KFKTPAKVGL PIGFSLPDCL QVVREVQYDF121SLEKKTIEWA EEIKKIEEAE REAECKIAEA EAKVNSKSGP EGDSKMSFSK THSTATMPPP181INPILASLQH NSILTPTRVS SSATKQKVLS PPHIKADFNL ADFECEEDPF DNLELKTIDE241KEELRNILVG TTGPIMAQLL DNNLPRGGSG SVLQDEEVLA SLERATLDFK PLHKPNGFIT301LPQLGNCEKM SLSSKVSLPP IPAVSNIKSL SFPKLDSDDS NQKTAKLAST FHSTSCLRNG361TFQNSLKPST QSSASELNGH HTLGLSALNL DSGTEMPALT SSQMPSLSVL SVCTEESSPP421NTGPTVTPPN FSVSQVPNMP SCPQAYSELQ MLSPSERQCV ETVVNMGYSY ECVLRAMKKK481GENIEQILDY LFAHGQLCEK GFDPLLVEEA LEMHQCSEEK MMEFLQLMSK FKEMGFELKD541IKEVLLLHNN DQDNALEDLM ARAGASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
566 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.