Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NADK2 / C5orf33 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119875-100
Catalog Number: LS-G119875
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5ORF33(NADK2) (NM_001085411), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C5orf33 | NADKD1 | NAD kinase domain containing 1
- Origin Species
- Human
- SwissProt
- Q4G0N4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTCYRGFLLG SCCRVAGGRA AALRGPGAGG PAARPRLGGD GGGRRHLGQG QPRELAGCGS61RADGGFRPSR VVVVAKTTRY EFEQQRYRYA ELSEEDLKQL LALKGSSYSG LLERHHIHTK121NVEHIIDSLR NEGIEVRLVK RREYDEETVR WADAVIAAGG DGTMLLAASK VLDRLKPVIG181VNTDPERSEG HLCLPVRYTH SFPEALQKFY RGEFRWLWRQ RIRLYLEGTG INPVPVDLHE241QQLSLNQHNR ALNIERAHDE RSEASGPQLL PVRALNEVFI GESLSSRASY YEISVDDGPW301EKQKSSGLNL CTGTGSKAWS FNINRVATQA VEDVLNIAKR QGNLSLPLNR ELVEKVTNEY361NESLLYSPEE PKILFSIREP IANRVFSSSR QRCFSSKVCV RSRCWDACMV VDGGTSFEFN421DGAIASMMIN KEDELRTVLL EQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
442 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.