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NAB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

NAB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all NAB2 antibodies →
$504.00each
Catalog Number: LS-G115613-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NAB2 (NM_005967), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EGR1 binding protein 2 | MADER | NAB2 | Protein MADER | EGR-1-binding protein 2 | NGFI-A-binding protein 2
Origin Species
Human
NCBI GenBank
NM_005967
SwissProt
Q15742

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ RANLLSYYET
61FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV
121PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG
181DPRIWPGRST PESDVGAGGE EEAGSPPFSP PAGGGVPEGT GAGGLAAGGT GGGPDRLEPE
241MVRMVVESVE RIFRSFPRGD AGEVTSLLKL NKKLARSVGH IFEMDDNDSQ KEEEIRKYSI
301IYGRFDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL
361KGSRLHPEEL GGPPLKKLKQ EVGEQSHPEI QQPPPGPESY VPPYRPSLEE DSASLSGESL
421DGHLQAVGSC PRLTPPPADL PLALPAHGLW SRHILQQTLM DEGLRLARLV SHDRVGRLSP
481CVPAKPPLAE FEEGLLDRCP APGPHPALVE GRRSSVKVEA EASRQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

525 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.