Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NAALADL / NAALADL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115949-100
Catalog Number: LS-G115949
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NAALADL1 (NM_005468), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Ileal peptidase | Ileal peptidase I100 | NAALADase L | NAALADL1 | I100 | Ileal dipeptidylpeptidase | NAALADL | NAALADASEL
- Origin Species
- Human
- NCBI GenBank
- NM_005468
- SwissProt
- Q9UQQ1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQWTKVLGLG LGAAALLGLG IILGHFAIPK KANSLAPQDL DLEILETVMG QLDAHRIREN61LRELSREPHL ASSPRDEDLV QLLLQRWKDP ESGLDSAEAS TYEVLLSFPS QEQPNVVDIV121GPTGGIIHSC HRTEENVTGE QGGPDVVQPY AAYAPSGTPQ GLLVYANRGA EEDFKELQTQ181GIKLEGTIAL TRYGGVGRGA KAVNAAKHGV AGVLVYTDPA DINDGLSSPD ETFPNSWYLP241PSGVERGSYY EYFGDPLTPY LPAVPSSFRV DLANVSGFPP IPTQPIGFQD ARDLLCNLNG301TLAPATWQGA LGCHYRLGPG FRPDGDFPAD SQVNVSVYNR LELRNSSNVL GIIRGAVEPD361RYVLYGNHRD SWVHGAVDPS SGTAVLLELS RVLGTLLKKG TWRPRRSIVF ASWGAEEFGL421IGSTEFTEEF FNKLQERTVA YINVDISVFA NATLRVQGTP PVQSVVFSAT KEIRSPGPGD481LSIYDNWIRY FNRSSPVYGL VPSLGSLGAG SDYAPFVHFL GISSMDIAYT YDRSKTSARI541YPTYHTAFDT FDYVDKFLDP GFSSHQAVAR TAGSVILRLS DSFFLPLKVS DYSETLRSFL601QAAQQDLGAL LEQHSISLGP LVTAVEKFEA EAAALGQRIS TLQKGSPDPL QVRMLNDQLM661LLERTFLNPR AFPEERYYSH VLWAPRTGSV VTFPGLSNAC SRARDTASGS EAWAEVQRQL721SIVVTALEGA AATLRPVADLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
740 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.