Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
NAA16 / NARG1L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101885-100
Catalog Number: LS-G101885
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NARG1L(NAA16) (NM_024561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NARG1L | NMDA receptor regulated 1-like | RP11-396A22.1 | NAA16 | NARG1-like protein | PRO2435
- Origin Species
- Human
- NCBI GenBank
- NM_024561
- SwissProt
- Q6N069
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPNVLLPPKE SNLFKRILKC YEQKQYKNGL KFCKMILSNP KFAEHGETLA MKGLTLNCLG61KKEEAYEFVR KGLRNDVKSH VCWHVYGLLQ RSDKKYDEAI KCYRNALKLD KDNLQILRDL121SLLQIQMRDL EGYRETRYQL LQLRPTQRAS WIGYAIAYHL LKDYDMALKL LEEFRQTQQV181PPNKIDYEYS ELILYQNQVM READLLQESL EHIEMYEKQI CDKLLVEEIK GEILLKLGRL241KEASEVFKNL IDRNAENWCY YEGLEKALQI STLEERLQIY EEISKQHPKA ITPRRLPLTL301VPGERFRELM DKFLRVNFSK GCPPLFTTLK SLYYNTEKVS IIQELVTNYE ASLKTCDFFS361PYENGEKEPP TTLLWVQYFL AQHFDKLGQY SLALDYINAA IASTPTLIEL FYMKAKIYKH421IGNLKEAAKW MDEAQSLDTA DRFINSKCAK YMLRANMIKE AEEMCSKFTR EGTSAMENLN481EMQCMWFQTE CISAYQRLGR YGDALKKCHE VERHFFEITD DQFDFHTYCM RKMTLRAYVD541LLRLEDILRR HAFYFKAARS AIEIYLKLYD NPLTNESKQQ EINSENLSAK ELKKMLSKQR601RAQKKAKLEE ERKHAERERQ QKNQKKKRDE EEEEASGLKE ELIPEKLERV ENPLEEAVKF661LIPLKNLVAD NIDTHLLAFE IYFRKGKFLL MLQSVKRAFA INSNNPWLHE CLIRFSKSVS721NHSNLPDIVS KVLSQEMQKI FVKKDLESFN EDFLKRNATS LQHLLSGAKM MYFLDKSRQE781KAIAIATRLD ETIKDKDVKT LIKVSEALLD GSFGNCSSQY EEYRMACHNL LPFTSAFLPA841VNEVDNPNVA LNHTANYDVL ANEITRTRPLEQKLISEEDLAANDILDYKDDDDKV
864 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.