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N4BP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

N4BP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113466-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human N4BP3 (NM_015111), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0341 | LZTS4 | NEDD4-binding protein 3 | N4BP3 | NEDD4 binding protein 3
Origin Species
Human
NCBI GenBank
NM_015111

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MATAPGPAGI AMGSVGSLLE RQDFSPEELR AALAGSRGSR QPDGLLRKGL GQREFLSYLH
61LPKKDSKSTK NTKRAPRNEP ADYATLYYRE HSRAGDFSKT SLPERGRFDK CRIRPSVFKP
121TAGNGKGFLS MQSLASHKGQ KLWRSNGSLH TLACHPPLSP GPQASQARAQ LLHALSLDEG
181GPEPEPSLSD SSSGGSFGRS PGTGPSPFSS SLGHLNHLGG SLDRASQGPK EAGPPAVLSC
241LPEPPPPYEF SCSSAEEMGA VLPETCEELK RGLGDEDGSN PFTQVLEERQ RLWLAELKRL
301YVERLHEVTQ KAERSERNLQ LQLFMAQQEQ RRLRKELRAQ QGLAPEPRAP GTLPEADPSA
361RPEEEARWEV CQKTAEISLL KQQLREAQAE LAQKLAEIFS LKTQLRGSRA QAQAQDAELV
421RLREAVRSLQ EQAPREEAPG SCETDDCKSR GLLGEAGGSE ARDSAEQLRA ELLQERLRGQ
481EQALRFEQER RTWQEEKERV LRYQREIQGG YMDMYRRNQA LEQELRALRE PPTPWSPR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

538 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.