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N4BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

N4BP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111774-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human N4BP2 (NM_018177), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_018177

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
198.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPRRRKNLGG NPFRKTANPK EVVVSSVASR EEPTTTLPSM GETKVDQEEL FTSISEIFSD
61LDPDVVYLML SECDFKVENA MDCLLELSAT DTKIEESSSQ SFVASENQVG AAESKIMKKR
121PEEESEDSKM DSFLDMQLTE DLDSLIQNAF EKLNSSPDDQ VYSFLPSQDV NSFNDSSEFI
181NPDSSNMTPI FSTQNMNLNG ENLENSGSTL SLNPLPSHSV LNESKCFIKD NTLALESNYP
241EDSLLSSSLN VASDSIAGCS SLNQKQKELL ESECVEAQFS EAPVDLDASE PQACLNLPGL
301DLPGTGGDQK STRVSDVFLP SEGFNFKPHK HPELPTKGKD VSYCPVLAPL PLLLPPPPPP
361PMWNPMIPAF DLFQGNHGFV APVVTTAAHW RSVNYTFPPS VISHTSPTKV WRNKDGTSAY
421QVQETPVSQV VRKKTSYVGL VLVLLRGLPG SGKSFLARTL QEDNPSGVIL STDDYFYING
481QYQFDVKYLG EAHEWNQNRA KEAFEKKISP IIIDNTNLQA WEMKPYVALS QKHKYKVLFR
541EPDTWWKFKP KELARRNIHG VSKEKITRML EHYQRFVSVP IIMSSSVPEK IERIELCAYS
601CEDRSTSPRD NEDIISEKEE NILSLSLKHL EFTEEKNLDV TKETMLPENV AYLSNADLNK
661RRKEISDMNP SIQSALILET PHMYFSDSES KLQATDKSEN EQIEMVAVKG YSKTDTDSSM
721ERVSPSTCCS ENNQEDCDLA NSGPLQNEKS SPGEIVEERA TVTKKAFGKQ KSKSTLEKFP
781RHELSNFVGD WPVDKTIGQR TKRNRKTEKT SSVQSDKKYN YPQSHKLVNS VSVNTDCVQQ
841RGSPHESVED GRKSQCDDAS EPLNSYKYDA YKNIDKNSFN IMGDWPSSDS LAQREHRSRM
901PKTGLSEPNL EIGTNDKMNE ISLSTAHEAC WGTSSQKLKT LGSSNLGSSE MLLSEMTCES
961QTCLSKKSHG QHTSLPLTFT NSAPTVSGVV EPQTLAECQE QMPKRDPGKE VGMCTQTEPQ
1021DFALLWKIEK NKISISDSIK VLTGRLDGFK PKVFNINTKS DVQEAIPYRV MYDKSTFVEE
1081SELTSADESE NLNILCKLFG SFSLEALKDL YERCNKDIIW ATSLLLDSET KLCEDTEFEN
1141FQKSCDGSQI GPFSLGLNLK EIISQRGTLE NSNSPVPEFS HGIGISNADS QSTCDAERGN
1201SEQAEMRAVT PENHESMTSI FPSAAVGLKN NNDILPNSQE ELLYSSKQSF PGILKATTPK
1261DMSETEKNLV VTETGDNIHS PSHFSDIFNF VSSTSNLELN EEIYFTDSLE IKRNENFPKD
1321YVKFSDEEEF MNEDEKEMKE ILMAGSSLSA GVRGEDKTEI LNPTPAMAKS LTIDCLELAL
1381PPELAFQLNE LFGPVGIDSG SLTVEDCVVH IDLNLAKVIH EKWKESVMER QRQEEVSCGK
1441FMQDPSLVGH TGLDNPEQKS SQRTGKKLLK TLTASEMLPL LDHWNTQTKK VSLREIMSEE
1501IALQEKHNLK RETLMFEKDC ATKLKEKQLF KIFPAINQNF LVDIFKDHNY SLEHTVQFLN
1561CVLEGDPVKT VVAQEFVHQN ENVTSHTGQK SKEKKPKKLK ETEETPSELS FQDFEYPDYD
1621DYRAEAFLHQ QKRMECYSKA KEAYRIGKKN VATFYAQQGT LHEQKMKEAN HLAAIEIFEK
1681VNASLLPQNV LDLHGLHVDE ALEHLMRVLE KKTEEFKQNG GKPYLSVITG RGNHSQGGVA
1741RIKPAVIKYL ISHSFRFSEI KPGCLKVMLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1770 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.