Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MZF / MZF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117370-100
Catalog Number: LS-G117370
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Myeloid zinc finger 1(MZF1) (NM_003422), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MZF-1 | MZF1B | MZF1 | Zinc finger protein 42 | ZSCAN6 | ZFP98 | Myeloid zinc finger 1 | MZF | ZNF42
- Origin Species
- Human
- NCBI GenBank
- NM_003422
- SwissProt
- P28698
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 81.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRPAVLGSPD RAPPEDEGPV MVKLEDSEEE GEAALWDPGP EAARLRFRCF RYEEATGPQE61ALAQLRELCR QWLRPEVRSK EQMLELLVLE QFLGALPPEI QARVQGQRPG SPEEAAALVD121GLRREPGGPR RWVTVQVQGQ EVLSEKMEPS SFQPLPETEP PTPEPGPKTP PRTMQESPLG181LQVKEESEVT EDSDFLESGP LAATQESVPT LLPEEAQRCG TVLDQIFPHS KTGPEGPSWR241EHPRALWHEE AGGIFSPGFA LQLGSISAGP GSVSPHLHVP WDLGMAGLSG QIQSPSREGG301FAHALLLPSD LRSEQDPTDE DPCRGVGPAL ITTRWRSPRG RSRGRPSTGG GVVRGGRCDV361CGKVFSQRSN LLRHQKIHTG ERPFVCSECG RSFSRSSHLL RHQLTHTEER PFVCGDCGQG421FVRSARLEEH RRVHTGEQPF RCAECGQSFR QRSNLLQHQR IHGDPPGPGA KPPAPPGAPE481PPGPFPCSEC RESFARRAVL LEHQAVHTGD KSFGCVECGE RFGRRSVLLQ HRRVHSGERP541FACAECGQSF RQRSNLTQHR RIHTGERPFA CAECGKAFRQ RPTLTQHLRV HTGEKPFACP601ECGQRFSQRL KLTRHQRTHT GEKPYHCGEC GLGFTQVSRL TEHQRIHTGE RPFACPECGQ661SFRQHANLTQ HRRIHTGERP YACPECGKAF RQRPTLTQHL RTHRREKPFA CQDCGRRFHQ721STKLIQHQRV HSAESGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
734 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.