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MYT1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYT1L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYT1L antibodies →
$725.00each
Catalog Number: LS-G113497-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYT1L (NM_015025), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MyT1-L | KIAA1106 | MYT1L | NZF1 | ZC2HC4B
Origin Species
Human
NCBI GenBank
AB029029
SwissProt
Q9UL68

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVDTEEKRH RTRSKGVRVP VEPAIQELFS CPTPGCDGSG HVSGKYARHR SVYGCPLAKK
61RKTQDKQPQE PAPKRKPFAV KADSSSVDEC DDSDGTEDMD EKEEDEGEEY SEDNDEPGDE
121DEEDEEGDRE EEEEIEEEDE DDDEDGEDVE DEEEEEEEEE EEEEEEENED HQMNCHNTRI
181MQDTEKDDNN NDEYDNYDEL VAKSLLNLGK IAEDAAYRAR TESEMNSNTS NSLEDDSDKN
241ENLGRKSELS LDLDSDVVRE TVDSLKLLAQ GHGVVLSENM NDRNYADSMS QQDSRNMNYV
301MLGKPMNNGL MEKMVEESDE EVCLSSLECL RNQCFDLARK LSETNPQERN PQQNMNIRQH
361VRPEEDFPGR TPDRNYSDML NLMRLEEQLS PRSRVFASCA KEDGCHERDD DTTSVNSDRS
421EEVFDMTKGN LTLLEKAIAL ETERAKAMRE KMAMEAGRRD NMRSYEDQSP RQLPGEDRKP
481KSSDSHVKKP YYDPSRTEKK ESKCPTPGCD GTGHVTGLYP HHRSLSGCPH KDRVPPEILA
541MHESVLKCPT PGCTGRGHVN SNRNSHRSLS GCPIAAAEKL AKAQEKHQSC DVSKSSQASD
601RVLRPMCFVK QLEIPQYGYR NNVPTTTPRS NLAKELEKYS KTSFEYNSYD NHTYGKRAIA
661PKVQTRDISP KGYDDAKRYC KDPSPSSSST SSYAPSSSSN LSCGGGSSAS STCSKSSFDY
721THDMEAAHMA ATAILNLSTR CREMPQNLST KPQDLCATRN PDMEVDENGT LDLSMNKQRP
781RDSCCPILTP LEPMSPQQQA VMNNRCFQLG EGDCWDLPVD YTKMKPRRID EDESKDITPE
841DLDPFQEALE ERRYPGEVTI PSPKPKYPQC KESKKDLITL SGCPLADKSI RSMLATSSQE
901LKCPTPGCDG SGHITGNYAS HRSLSGCPRA KKSGIRIAQS KEDKEDQEPI RCPVPGCDGQ
961GHITGKYASH RSASGCPLAA KRQKDGYLNG SQFSWKSVKT EGMSCPTPGC DGSGHVSGSF
1021LTHRSLSGCP RATSAMKKAK LSGEQMLTIK QRASNGIEND EEIKQLDEEI KELNESNSQM
1081EADMIKLRTQ ITTMESNLKT IEEENKVIEQ QNESLLHELA NLSQSLIHSL ANIQLPHMDP
1141INEQNFDAYV TTLTEMYTNQ DRYQSPENKA LLENIKQAVR GIQVTRR
LEQKLISEEDLAANDILDYKDDDDKV

1187 aa + 26 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.