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MYRIP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYRIP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYRIP antibodies →
$725.00each
Catalog Number: LS-G113202-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYRIP (NM_015460), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Rab effector MyRIP | SLAC2-C | SLAC2C | MYRIP | Exophilin-8
Origin Species
Human
NCBI GenBank
NM_015460
SwissProt
Q8NFW9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRKLDLSGL TDDETEHVLQ VVQRDFNLRK KEEERLSELK QKLDEEGSKC SILSKHQQFV
61EHCCMRCCSP FTFLVNTKRQ CGDCKFNVCK SCCSYQKHEK AWVCCVCQQA RLLRAQSLEW
121FYNNVKSRFK RFGSAKVLKN LYRKHRLESG ACFDILGGSL FESNLENEGS ISGSDSTFYR
181QSEGHSVMDT LAVALRVAEE AIEEAISKAE AYGDSLDKQN EASYLRDHKE ELTEELATTI
241LQKIIRKQKS KSEQQVEEEP GWPHPQSCST KVADEGTSAS PGGYRAPAAL WRSQSAFSIT
301GEEALKTPPV EAPSRQPRDQ GQHPRAESAL PSWKSVDRLD ETNLAPVLQS PDGNWVALKD
361GAPPPTRLLA KPKSGTFQAL EVASSVASAY DEMGSDSEED FDWSEALSKL CPRSRALPRN
421PQPQPTQAQS SDQGPIAASP SSALSPNPEA MCSDSETSSA GSSREFGHQA RLSWLQRKAP
481RNPAAEKMRL HGELDVNFNP QLASRETSDS SEPEEAPHTT DRRARRWRRA RLGSEEPSKE
541PSSPSAQLRD LDTHQVSDDL SETDISNEAR DPQTLTDTTE EKRRNRLYEL AMKMSEKETS
601SGEDQESEPK TESENQKESL SSEDNSQSVQ EELKKKFSAV SLCNISTEVL KVINATEELI
661AGSTGPWESP QVPPDRQKGM FPRGTDQVRL DEQLTSLEEN VYLAAGTVYG LETQLTELED
721AARCIHSGTD ETHLADLEDQ VATAAAQVHH AELQISDIES RISALTIAGL NIAPCVRFTR
781RRDQKQRTQV QTIDTSRQQR RKLPAPPVKA EKIETSSVTT IKTFNHNFIL QGSSTNRTKE
841RKGTTKDLME PALESAVMY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

859 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.