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MYOM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYOM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYOM3 antibodies →
$725.00each
Catalog Number: LS-G106342-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYOM3 (NM_152372), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
162 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTLPHSLGGA GDPRPPQAME VHRLEHRQEE EQKEERQHSL RMGSSVRRRT FRSSEEEHEF
61SAADYALAAA LALTASSELS WEAQLRRQTS AVELEERGQK RVGFGNDWER TEIAFLQTHR
121LLRQRRDWKT LRRRTEEKVQ EAKELRELCY GRGPWFWIPL RSHAVWEHTT VLLTCTVQAS
181PPPQVTWYKN DTRIDPRLFR AGKYRITNNY GLLSLEIRRC AIEDSATYTV RVKNAHGQAS
241SFAKVLVRTY LGKDAGFDSE IFKRSTFGPS VEFTSVLKPV FAREKEPFSL SCLFSEDVLD
301AESIQWFRDG SLLRSSRRRK ILYTDRQASL KVSCTYKEDE GLYMVRVPSP FGPREQSTYV
361LVRDAEAENP GAPGSPLNVR CLDVNRDCLI LTWAPPSDTR GNPITAYTIE RCQGESGEWI
421ACHEAPGGTC RCPIQGLVEG QSYRFRVRAI SRVGSSVPSK ASELVVMGDH DAARRKTEIP
481FDLGNKITIS TDAFEDTVTI PSPPTNVHAS EIREAYVVLA WEEPSPRDRA PLTYSLEKSV
541IGSGTWEAIS SESPVRSPRF AVLDLEKKKS YVFRVRAMNQ YGLSDPSEPS EPIALRGPPA
601TLPPPAQVQA FRDTQTSVSL TWDPVKDPEL LGYYIYSRKV GTSEWQTVNN KPIQGTRFTV
661PGLRTGKEYE FCVRSVSEAG VGESSAATEP IRVKQALATP SAPYGFALLN CGKNEMVIGW
721KPPKRRGGGK ILGYFLDQHD SEELDWHAVN QQPIPTRVCK VSDLHEGHFY EFRARAANWA
781GVGELSAPSS LFECKEWTMP QPGPPYDVRA SEVRATSLVL QWEPPLYMGA GPVTGYHVSF
841QEEGSEQWKP VTPGPISGTH LRVSDLQPGK SYVFQVQAMN SAGLGQPSMP TDPVLLEDKP
901GAHEIEVGVD EEGFIYLAFE APEAPDSSEF QWSKDYKGPL DPQRVKIEDK VNKSKVILKE
961PGLEDLGTYS VIVTDADEDI SASHTLTEEE LEKLKKLSHE IRNPVIKLIS GWNIDILERG
1021EVRLWLEVEK LSPAAELHLI FNNKEIFSSP NRKINFDREK GLVEVIIQNL SEEDKGSYTA
1081QLQDGKAKNQ ITLTLVDDDF DKLLRKADAK RRDWKRKQGP YFERPLQWKV TEDCQVQLTC
1141KVTNTKKETR FQWFFQRAEM PDGQYDPETG TGLLCIEELS KKDKGIYRAM VSDDRGEDDT
1201ILDLTGDALD AIFTELGRIG ALSATPLKIQ GTEEGIRIFS KVKYYNVEYM KTTWFHKDKR
1261LESGDRIRTG TTLDEIWLHI LDPKDSDKGK YTLEIAAGKE VRQLSTDLSG QAFEDAMAEH
1321QRLKTLAIIE KNRAKVVRGL PDVATIMEDK TLCLTCIVSG DPTPEISWLK NDQPVTFLDR
1381YRMEVRGTEV TITIEKVNSE DSGRYGVFVK NKYGSETGQV TISVFKHGDE PKELKSM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1437 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.