Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MYOM1 / Myomesin 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117080-100
Catalog Number: LS-G117080
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYOM1 (NM_003803), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EH-myomesin | Myomesin 1 (skelemin) 185kDa | MYOM1 | Myomesin (M-protein) 1 (190kD) | Myomesin 1 (skelemin) (185kD) | Myomesin 1 | 185kDa | Myomesin family member 1 | Myomesin-1 | SKELEMIN | Myomesin
- Origin Species
- Human
- NCBI GenBank
- NM_003803
- SwissProt
- P52179
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 187.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLPFYQRCH QHYDLSYRNK DVRSTVSHYQ REKKRSAVYT QGSTAYSSRS SAAHRRESEA61FRRASASSSQ QQASQHALSS EVSRKAASAY DYGSSHGLTD SSLLLDDYSS KLSPKPKRAK121HSLLSGEEKE NLPSDYMVPI FSGRQKHVSG ITDTEEERIK EAAAYIAQRN LLASEEGITT181PKQSTASKQT TASKQSTASK QSTASKQSTA SRQSTASRQS VVSKQATSAL QQEETSEKKS241RKVVIREKAE RLSLRKTLEE TETYHAKLNE DHLLHAPEFI IKPRSHTVWE KENVKLHCSI301AGWPEPRVTW YKNQVPINVH ANPGKYIIES RYGMHTLEIN ACDFEDTAQY RASAMNVKGE361LSAYASVVVK RYKGEFDETR FHAGASTMPL SFGVTPYGYA SRFEIHFDDK FDVSFGREGE421TMSLGCRVVI TPEIKHFQPE IQWYRNGVPL SPSKWVQTLW SGERATLTFS HLNKEDEGLY481TIRVRMGEYY EQYSAYVFVR DADAEIEGAP AAPLDVKCLE ANKDYIIISW KQPAVDGGSP541ILGYFIDKCE VGTDSWSQCN DTPVKFARFP VTGLIEGRSY IFRVRAVNKM GIGFPSRVSE601PVAALDPAEK ARLKSRPSAP WTGQIIVTEE EPSEGIVPGP PTDLSVTEAT RSYVVLSWKP661PGQRGHEGIM YFVEKCEAGT ENWQRVNTEL PVKSPRFALF DLAEGKSYCF RVRCSNSAGV721GEPSEATEVT VVGDKLDIPK APGKIIPSRN TDTSVVVSWE ESKDAKELVG YYIEASVAGS781GKWEPCNNNP VKGSRFTCHG LVTGQSYIFR VRAVNAAGLS EYSQDSEAIE VKAAIGGGVS841PDVCPALSDE PGGLTASRGR VHEASPPTFQ KDALLGSKPN KPSLPSSSQN LGQTEVSKVS901ETVQEELTPP PQKAAPQGKS KSDPLKKKTD RAPPSPPCDI TCLESFRDSM VLGWKQPDKI961GGAEITGYYV NYREVIDGVP GKWREANVKA VSEEAYKISN LKENMVYQFQ VAAMNMAGLG1021APSAVSECFK CEEWTIAVPG PPHSLKCSEV RKDSLVLQWK PPVHSGRTPV TGYFVDLKEA1081KAKEDQWRGL NEAAIKNVYL KVRGLKEGVS YVFRVRAINQ AGVGKPSDLA GPVVAETRPG1141TKEVVVNVDD DGVISLNFEC DKMTPKSEFS WSKDYVSTED SPRLEVESKG NKTKMTFKDL1201GMDDLGIYSC DVTDTDGIAS SYLIDEEELK RLLALSHEHK FPTVPVKSEL AVEILEKGQV1261RFWMQAEKLS GNAKVNYIFN EKEIFEGPKY KMHIDRNTGI IEMFMEKLQD EDEGTYTFQL1321QDGKATNHST VVLVGDVFKK LQKEAEFQRQ EWIRKQGPHF VEYLSWEVTG ECNVLLKCKV1381ANIKKETHIV WYKDEREISV DEKHDFKDGI CTLLITEFSK KDAGIYEVIL KDDRGKDKSR1441LKLVDEAFKE LMMEVCKKIA LSATDLKIQS TAEGIQLYSF VTYYVEDLKV NWSHNGSAIR1501YSDRVKTGVT GEQIWLQINE PTPNDKGKYV MELFDGKTGH QKTVDLSGQA YDEAYAEFQR1561LKQAAIAEKN RARVLGGLPD VVTIQEGKAL NLTCNVWGDP PPEVSWLKNE KALASDDHCN1621LKFEAGRTAY FTINGVSTAD SGKYGLVVKN KYGSETSDFT VSVFIPEEEA RMAALESLKG1681GKKAKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1685 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.