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MYO1F Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYO1F Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYO1F antibodies →
$504.00each
Catalog Number: LS-G114497-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYO1F (NM_012335), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MYO1F | Myosin-ID | Unconventional myosin-If | Myosin IF | Myosin-Ie
Origin Species
Human
NCBI GenBank
X98411
SwissProt
O00160

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSKERFHWQ SHNVKQSGVD DMVLLPQITE DAIAANLRKR FMDDYIFTYI GSVLISVNPF
61KQMPYFTDRE IDLYQGAAQY ENPPHIYALT DNMYRNMLID CENQCVIISG ESGAGKTVAA
121KYIMGYISKV SGGGEKVQHV KDIILQSNPL LEAFGNAKTV RNNNSSRFGK YFEIQFSRGG
181EPDGGKISNF LLEKSRVVMQ NENERNFHIY YQLLEGASQE QRQNLGLMTP DYYYYLNQSD
241TYQVDGTDDR SDFGETLSAM QVIGIPPSIQ QLVLQLVAGI LHLGNISFCE DGNYARVESV
301DLLAFPAYLL GIDSGRLQEK LTSRKMDSRW GGRSESINVT LNVEQAAYTR DALAKGLYAR
361LFDFLVEAIN RAMQKPQEEY SIGVLDIYGF EIFQKNGFEQ FCINFVNEKL QQIFIELTLK
421AEQEEYVQEG IRWTPIQYFN NKVVCDLIEN KLSPPGIMSV LDDVCATMHA TGGGADQTLL
481QKLQAAVGTH EHFNSWSAGF VIHHYAGKVS YDVSGFCERN RDVLFSDLIE LMQTSEQAFL
541RMLFPEKLDG DKKGRPSTAG SKIKKQANDL VATLMRCTPH YIRCIKPNET KRPRDWEENR
601VKHQVEYLGL KENIRVRRAG FAYRRQFAKF LQRYAILTPE TWPRWRGDER QGVQHLLRAV
661NMEPDQYQMG STKVFVKNPE SLFLLEEVRE RKFDGFARTI QKAWRRHVAV RKYEEMREEA
721SNILLNKKER RRNSINRNFV GDYLGLEERP ELRQFLGKRE RVDFADSVTK YDRRFKPIKR
781DLILTPKCVY VIGREKVKKG PEKGQVCEVL KKKVDIQALR GVSLSTRQDD FFILQEDAAD
841SFLESVFKTE FVSLLCKRFE EATRRPLPLT FSDTLQFRVK KEGWGGGGTR SVTFSRGFGD
901LAVLKVGGRT LTVSVGDGLP KSSKPTRKGM AKGKPRRSSQ APTRAAPAPP RGMDRNGVPP
961SARGGPLPLE IMSGGGTHRP PRGPPSTSLG ASRRPRARPP SEHNTEFLNV PDQGMAGMQR
1021KRSVGQRPVP GVGRPKPQPR THGPRCRALY QYVGQDVDEL SFNVNEVIEI LMEDPSGWWK
1081GRLHGQEGLF PGNYVEKI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1098 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.