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MYO1C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYO1C Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYO1C antibodies →
$504.00each
Catalog Number: LS-G102130-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Myosin 1C(MYO1C) (NM_033375), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MMI-beta | MMIb | MYO1C | Myosin-I beta | Myosin IC | Myosin-IC | Unconventional myosin-Ic | Nuclear myosin I | Myosin I beta | Myr2
Origin Species
Human
NCBI GenBank
X98507
SwissProt
O00159

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
117.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESALTARDR VGVQDFVLLE NFTSEAAFIE NLRRRFRENL IYTYIGPVLV SVNPYRDLQI
61YSRQHMERYR GVSFYEVPPH LFAVADTVYR ALRTERRDQA VMISGESGAG KTEATKRLLQ
121FYAETCPAPE RGGAVRDRLL QSNPVLEAFG NAKTLRNDNS SRFGKYMDVQ FDFKGAPVGG
181HILSYLLEKS RVVHQNHGER NFHIFYQLLE GGEEETLRRL GLERNPQSYL YLVKGQCAKV
241SSINDKSDWK VVRKALTVID FTEDEVEDLL SIVASVLHLG NIHFAANEES NAQVTTENQL
301KYLTRLLSVE GSTLREALTH RKIIAKGEEL LSPLNLEQAA YARDALAKAV YSRTFTWLVG
361KINRSLASKD VESPSWRSTT VLGLLDIYGF EVFQHNSFEQ FCINYCNEKL QQLFIELTLK
421SEQEEYEAEG IAWEPVQYFN NKIICDLVEE KFKGIISILD EECLRPGEAT DLTFLEKLED
481TVKHHPHFLT HKLADQRTRK SLGRGEFRLL HYAGEVTYSV TGFLDKNNDL LFRNLKETMC
541SSKNPIMSQC FDRSELSDKK RPETVATQFK MSLLQLVEIL QSKEPAYVRC IKPNDAKQPG
601RFDEVLIRHQ VKYLGLLENL RVRRAGFAYR RKYEAFLQRY KSLCPETWPT WAGRPQDGVA
661VLVRHLGYKP EEYKMGRTKI FIRFPKTLFA TEDALEVRRQ SLATKIQAAW RGFHWRQKFL
721RVKRSAICIQ SWWRGTLGRR KAAKRKWAAQ TIRRLIRGFI LRHAPRCPEN AFFLDHVRTS
781FLLNLRRQLP RNVLDTSWPT PPPALREASE LLRELCIKNM VWKYCRSISP EWKQQLQQKA
841VASEIFKGKK DNYPQSVPRL FISTRLGTDE ISPRVLQALG SEPIQYAVPV VKYDRKGYKP
901RSRQLLLTPN AVVIVEDAKV KQRIDYANLT GISVSSLSDS LFVLHVQRAD NKQKGDVVLQ
961SDHVIETLTK TALSANRVNS ININQGSITF AGGPGRDGTI DFTPGSELLI TKAKNGHLAV
1021VAPRLNSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1028 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.