LSBio
MYH3 Protein (Over-Expression Lysate Myc-DDK (Flag))

MYH3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYH3 antibodies →
$725.00each
Catalog Number: LS-G99763-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human myosin heavy chain 3(MYH3) (NM_002470), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HEMHC | MYH3 | SMHCE | MYHC-EMB | MYHSE1 | Myosin heavy chain 3 | Myosin-3
NCBI GenBank
NM_002470
SwissProt
P11055

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
224.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSDTEMEVF GIAAPFLRKS EKERIEAQNQ PFDAKTYCFV VDSKEEYAKG KIKSSQDGKV
61TVETEDNRTL VVKPEDVYAM NPPKFDRIED MAMLTHLNEP AVLYNLKDRY TSWMIYTYSG
121LFCVTVNPYK WLPVYNPEVV EGYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE
181SGAGKTVNTK RVIQYFATIA ATGDLAKKKD SKMKGTLEDQ IISANPLLEA FGNAKTVRND
241NSSRFGKFIR IHFGTTGKLA SADIETYLLE KSRVTFQLKA ERSYHIFYQI LSNKKPELIE
301LLLITTNPYD YPFISQGEIL VASIDDAEEL LATDSAIDIL GFTPEEKSGL YKLTGAVMHY
361GNMKFKQKQR EEQAEPDGTE VADKTAYLMG LNSSDLLKAL CFPRVKVGNE YVTKGQTVDQ
421VHHAVNALSK SVYEKLFLWM VTRINQQLDT KLPRQHFIGV LDIAGFEIFE YNSLEQLCIN
481FTNEKLQQFF NHHMFVLEQE EYKKEGIEWT FIDFGMDLAA CIELIEKPMG IFSILEEECM
541FPKATDTSFK NKLYDQHLGK SNNFQKPKVV KGRAEAHFSL IHYAGTVDYS VSGWLEKNKD
601PLNETVVGLY QKSSNRLLAH LYATFATADA DSGKKKVAKK KGSSFQTVSA LFRENLNKLM
661SNLRTTHPHF VRCIIPNETK TPGAMEHSLV LHQLRCNGVL EGIRICRKGF PNRILYGDFK
721QRYRVLNASA IPEGQFIDSK KACEKLLASI DIDHTQYKFG HTKVFFKAGL LGTLEEMRDD
781RLAKLITRTQ AVCRGFLMRV EFQKMVQRRE SIFCIQYNIR SFMNVKHWPW MKLFFKIKPL
841LKSAETEKEM ATMKEEFQKT KDELAKSEAK RKELEEKLVT LVQEKNDLQL QVQAESENLL
901DAEERCDQLI KAKFQLEAKI KEVTERAEDE EEINAELTAK KRKLEDECSE LKKDIDDLEL
961TLAKVEKEKH ATENKVKNLT EELSGLDETI AKLTREKKAL QEAHQQALDD LQAEEDKVNS
1021LNKTKSKLEQ QVEDLESSLE QEKKLRVDLE RNKRKLEGDL KLAQESILDL ENDKQQLDER
1081LKKKDFEYCQ LQSKVEDEQT LGLQFQKKIK ELQARIEELE EEIEAERATR AKTEKQRSDY
1141ARELEELSER LEEAGGVTST QIELNKKREA EFLKLRRDLE EATLQHEAMV AALRKKHADS
1201VAELGEQIDN LQRVKQKLEK EKSEFKLEID DLSSSMESVS KSKANLEKIC RTLEDQLSEA
1261RGKNEEIQRS LSELTTQKSR LQTEAGELSR QLEEKESIVS QLSRSKQAFT QQTEELKRQL
1321EEENKAKNAL AHALQSSRHD CDLLREQYEE EQEGKAELQR ALSKANSEVA QWRTKYETDA
1381IQRTEELEEA KKKLAQRLQD SEEQVEAVNA KCASLEKTKQ RLQGEVEDLM VDVERANSLA
1441AALDKKQRNF DKVLAEWKTK CEESQAELEA SLKESRSLST ELFKLKNAYE EALDQLETVK
1501RENKNLEQEI ADLTEQIAEN GKTIHELEKS RKQIELEKAD IQLALEEAEA ALEHEEAKIL
1561RIQLELTQVK SEIDRKIAEK DEEIEQLKRN YQRTVETMQS ALDAEVRSRN EAIRLKKKME
1621GDLNEIEIQL SHANRQAAET LKHLRSVQGQ LKDTQLHLDD ALRGQEDLKE QLAIVERRAN
1681LLQAEVEELR ATLEQTERAR KLAEQELLDS NERVQLLHTQ NTSLIHTKKK LETDLMQLQS
1741EVEDASRDAR NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNLEQT VKDLQHRLDE
1801AEQLALKGGK KQIQKLETRI RELEFELEGE QKKNTESVKG LRKYERRVKE LTYQSEEDRK
1861NVLRLQDLVD KLQVKVKSYK RQAEEADEQA NAHLTKFRKA QHELEEAEER ADIAESQVNK
1921LRAKTRDFTS SRMVVHESEE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1940 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.