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MYEF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYEF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYEF2 antibodies →
$725.00each
Catalog Number: LS-G112834-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Myelin expression factor 2(MYEF2) (NM_016132), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1341 | MST156 | MyEF-2 | MEF-2 | MSTP156 | HsT18564 | MYEF2 | Myelin expression factor 2
Origin Species
Human
NCBI GenBank
NM_016132
SwissProt
Q9P2K5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADANKAEVP GATGGDSPHL QPAEPPGEPR REPHPAEAEK QQPQHSSSSN GVKMENDESA
61KEEKSDLKEK STGSKKANRF HPYSKDKNSG TGEKKGPNRN RVFISNIPYD MKWQAIKDLM
121REKVGEVTYV ELFKDAEGKS RGCGVVEFKD EEFVKKALET MNKYDLSGRP LNIKEDPDGE
181NARRALQRTG GSFPGGHVPD MGSGLMNLPP SILNNPNIPP EVISNLQAGR LGSTIFVANL
241DFKVGWKKLK EVFSIAGTVK RADIKEDKDG KSRGMGTVTF EQAIEAVQAI SMFNGQFLFD
301RPMHVKMDDK SVPHEEYRSH DGKTPQLPRG LGGIGMGLGP GGQPISASQL NIGGVMGNLG
361PGGMGMDGPG FGGMNRIGGG IGFGGLEAMN SMGGFGGVGR MGELYRGAMT SSMERDFGRG
421DIGINRGFGD SFGRLGSAMI GGFAGRIGSS NMGPVGSGIS GGMGSMNSVT GGMGMGLDRM
481SSSFDRMGPG IGAILERSID MDRGFLSGPM GSGMRERIGS KGNQIFVRNL PFDLTWQKLK
541EKFSQCGHVM FAEIKMENGK SKGCGTVRFD SPESAEKACR IMNGIKISGR EIDVRLDRNA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.