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MYCBPAP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYCBPAP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYCBPAP antibodies →
$725.00each
Catalog Number: LS-G129147-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYCBPAP (NM_032133), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
111.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRAPARGTGC CGRSGGRWLA GAAQPRCLWA GGAGQRFMVP GGTMKSLKKD SRLRITPTRL
61LEASENVKEK KRAKGPEQPT PTIQEEPEPV SNVLQGDDIL ALAIKKEDLK EQHIPRLTEK
121EDKRVITQKF IIRKLKPMDP RRKVCHLVAR PANPDEATKP LDYSGPGDSF DGSDQILPHH
181ILGSLQDFKR IALARGNTQL AERIPTSPCL MTLISAEGES KQKAPKEEKR PPWAPPPQHN
241FLKNWQRNTA LRKKQQEALS EHLKKPVSEL LMHTGETYRR IQEERELIDC TLPTRRDRKS
301WENSGFWSRL EYLGDEMTGL VMTKTKTQRG LMEPITHIRK PHSIRVETGL PAQRDASYRY
361TWDRSLFLIY RRKELQRIME ELDFSQQDID GLEVVGKGWP FSAVTVEDYT VFERSQGSSS
421EDTAYLGTLA SSSDVSMPIL GPSLLFCGKP ACWIRGSNPQ DKRQVGIAAH LTFETLEGEK
481TSSELTVVNN GTVAIWYDWR RQHQPDTFQD LKKNRMQRFY FDNREGVILP GEIKTFTFFF
541KSLTAGVFRE FWEFRTHPTL LGGAILQVNL HAVSLTQDVF EDERKVLESK LTAHEAVTVV
601REVLQELLMG VLTPERTPSP VDAYLTEEDL FRHRNPPLHY EHQVVQSLHQ LWRQYMTLPA
661KAEEARPGDK EHVSPIATEK ASVNAELLPR FRSPISETQV PRPENEALRE SGSQKARVGT
721KSPQRKSIME EILVEESPDV DSTKSPWEPD GLPLLEWNLC LEDFRKAVMV LPDENHREDA
781LMRLNKAALE LCQKPRPLQS NLLHQMCLQL WRDVIDSLVG HSMWLRSVLG LPEKETIYLN
841VPEEQDQKSP PIMEVKVPVG KAGKEERKGA AQEKKQLGIK DKEDKKGAKL LGKEDRPNSK
901KHKAKDDKKV IKSASQDRFS LEDPTPDIIL SSQEPIDPLV MGKYTQSLHS EVRGLLDTLV
961TDLMVLADEL SPIKNVEEAL RLCR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

984 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.