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MYBPHL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYBPHL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYBPHL antibodies →
$504.00each
Catalog Number: LS-G121764-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYBPHL (NM_001010985), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Myosin binding protein H-like | MYBPHL | Myosin-binding protein H-like
Origin Species
Human
NCBI GenBank
NM_001010985
SwissProt
A2RUH7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MEAATAPEVA AGSKLKVKEA SPADAEPPQA SPGQGAGSPT PQLLPPIEEH PKIWLPRALR
61QTYIRKVGDT VNLLIPFQGK PKPQAIWTHD GCALDTRRVS VRNGEQDSIL FIREAQRADS
121GRYQLRVQLG GLEATATIDI LVIERPGPPQ SIKLVDVWGF SATLEWTPPQ DTGNTALLGY
181TVQKADTKSG LWFTVLEHYH RTSCIVSDLI IGNSYAFRVF AENQCGLSET APITTDLAHI
241QKAATVYKTK GFAQRDFSEA PKFTQPLANC TTVTGYNTQL FCCVRASPRP KIIWLKNKMD
301IQGNPKYRAL THLGICSLEI RKPGPFDGGI YTCKAVNPLG EASVDCRVDV KVPN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

354 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.