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MYBPC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MYBPC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MYBPC1 antibodies →
$725.00each
Catalog Number: LS-G103006-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MYBPC1 (NM_206821), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MYBPCS | MYBPC1 | MYBPCC | LCCS4 | Slow MyBP-C | Slow-type | Skeletal muscle C-protein
Origin Species
Human
NCBI GenBank
NM_002465
SwissProt
Q00872

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPEPTKKEEN EVPAPAPPPE EPSKEKEAGT TPAKDWTLVE TPPGEEQAKQ NANSQLSILF
61IEKPQGGTVK VGEDITFIAK VKAEDLLRKP TIKWFKGKWM DLASKAGKHL QLKETFERHS
121RVYTFEMQII KAKDNFAGNY RCEVTYKDKF DSCSFDLEVH ESTGTTPNID IRSAFKRSGE
181GQEDAGELDF SGLLKRREVK QQEEEPQVDV WELLKNAKPS EYEKIAFQYG ITDLRGMLKR
241LKRMRREEKK SAAFAKILDP AYQVDKGGRV RFVVELADPK LEVKWYKNGQ EIRPSTKYIF
301EHKGCQRILF INNCQMTDDS EYYVTAGDEK CSTELFVREP PIMVTKQLED TTAYCGERVE
361LECEVSEDDA NVKWFKNGEE IIPGPKSRYR IRVEGKKHIL IIEGATKADA AEYSVMTTGG
421QSSAKLSVDL KPLKILTPLT DQTVNLGKEI CLKCEISENI PGKWTKNGLP VQESDRLKVV
481HKGRIHKLVI ANALTEDEGD YVFAPDAYNV TLPAKVHVID PPKIILDGLD ADNTVTVIAG
541NKLRLEIPIS GEPPPKAMWS RGDKAIMEGS GRIRTESYPD SSTLVIDIAE RDDSGVYHIN
601LKNEAGEAHA SIKVKVVDFP DPPVAPTVTE VGDDWCIMNW EPPAYDGGSP ILGYFIERKK
661KQSSRWMRLN FDLCKETTFE PKKMIEGVAY EVRIFAVNAI GISKPSMPSR PFVPLAVTSP
721PTLLTVDSVT DTTVTMRWRP PDHIGAAGLD GYVLEYCFEG TEDWIVANKD LIDKTKFTIT
781GLPTDAKIFV RVKAVNAAGA SEPKYYSQPI LVKEIIEPPK IRIPRHLKQT YIRRVGEAVN
841LVIPFQGKPR PELTWKKDGA EIDKNQINIR NSETDTIIFI RKAERSHSGK YDLQVKVDKF
901VETASIDIQI IDRPGPPQIV KIEDVWGENV ALTWTPPKDD GNAAITGYTI QKADKKSMEW
961FTVIEHYHRT SATITELVIG NEYYFRVFSE NMCGLSEDAT MTKESAVIAR DGKIYKNPVY
1021EDFDFSEAPM FTQPLVNTYA IAGYNATLNC SVRGNPKPKI TWMKNKVAIV DDPRYRMFSN
1081QGVCTLEIRK PSPYDGGTYC CKAVNDLGTV EIECKLEVKV IAQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1123 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.