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MX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117952-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MX2 (NM_002463), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MXB | MX dynamin-like GTPase 2 | interferon-induced GTP-binding protein Mx2 | myxovirus resistance protein 2 | interferon-regulated resistance GTP-binding protein MXB
Origin Species
Human
NCBI GenBank
NM_002463
SwissProt
P20592

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKAHKPWPY RRRSQFSSRK YLKKEMNSFQ QQPPPFGTVP PQMMFPPNWQ GAEKDAAFLA
61KDFNFLTLNN QPPPGNRSQP RAMGPENNLY SQYEQKVRPC IDLIDSLRAL GVEQDLALPA
121IAVIGDQSSG KSSVLEALSG VALPRGSGIV TRCPLVLKLK KQPCEAWAGR ISYRNTELEL
181QDPGQVEKEI HKAQNVMAGN GRGISHELIS LEITSPEVPD LTIIDLPGIT RVAVDNQPRD
241IGLQIKALIK KYIQRQQTIN LVVVPCNVDI ATTEALSMAH EVDPEGDRTI GILTKPDLMD
301RGTEKSVMNV VRNLTYPLKK GYMIVKCRGQ QEITNRLSLA EATKKEITFF QTHPYFRVLL
361EEGSATVPRL AERLTTELIM HIQKSLPLLE GQIRESHQKA TEELRRCGAD IPSQEADKMF
421FLIEKIKMFN QDIEKLVEGE EVVRENETRL YNKIREDFKN WVGILATNTQ KVKNIIHEEV
481EKYEKQYRGK ELLGFVNYKT FEIIVHQYIQ QLVEPALSML QKAMEIIQQA FINVAKKHFG
541EFFNLNQTVQ STIEDIKVKH TAKAENMIQL QFRMEQMVFC QDQIYSVVLK KVREEIFNPL
601GTPSQNMKLN SHFPSNESSV SSFTEIGIHL NAYFLETSKR LANQIPFIIQ YFMLRENGDS
661LQKAMMQILQ EKNRYSWLLQ EQSETATKRR ILKERIYRLT QARHALCQFS SKEIH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

715 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.