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MUM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MUM1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MUM1 antibodies →
$504.00each
Catalog Number: LS-G108582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MUM1 (NM_032853), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EXPAND1 | MUM1 | HSPC211 | MUM-1 | Protein expandere
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMADAKYVLC RWEKRLWPAK VLARTATSTK NKRRKEYFLA VQILSLEEKI KVKSTEVEIL
61EKSQIEAIAS SLASQNEVPA APLEELAYRR SLRVALDVLS EGSIWSQESS AGTGRADRSL
121RGKPMEHVSS PCDSNSSSLP RGDVLGSSRP HRRRPCVQQS LSSSFTCEKD PECKVDHKKG
181LRKSENPRGP LVLPAGGGAQ DESGSRIHHK NWTLASKRGR NSAQKASLCL NGSSLSEDDT
241ERDMGSKGGS WAAPSLPSGV REDDPCANAE GHDPGLPLGS LTAPPAPEPS ACSEPGECPA
301KKRPRLDGSQ RPPAVQLEPM AAGAAPSPGP GPGPRESVTP RSTARLGPPP SHASADATRC
361LPCPDSQKLE KECQSSEESM GSNSMRSILE EDEEDEEPPR VLLYHEPRSF EVGMLVWHKH
421KKYPFWPAVV KSVRQRDKKA SVLYIEGHMN PKMKGFTVSL KSLKHFDCKE KQTLLNQARE
481DFNQDIGWCV SLITDYRVRL GCGSFAGSFL EYYAADISYP VRKSIQQDVL GTKLPQLSKG
541SPEEPVVGCP LGQRQPCRKM LPDRSRAARD RANQKLVEYI VKAKGAESHL RAILKSRKPS
601RWLQTFLSSS QYVTCVETYL EDEGQLDLVV KYLQGVYQEV GAKVLQRTNG DRIRFILDVL
661LPEAIICAIS AVDEVDYKTA EEKYIKGPSL SYREKEIFDN QLLEERNRRR R
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

711 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.