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MTO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MTO1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114637-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MTO1 (NM_012123), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
COXPD10 | MTO1 | CGI-02 | Homolog of yeast Mto1 | Mitochondrial MTO1-3
Origin Species
Human
NCBI GenBank
NM_012123
SwissProt
Q9Y2Z2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFYFRGCGRW VAVSFTKQQF PLARLSSDSA APRTPHFDVI VIGGGHAGTE AATAAARCGS
61RTLLLTHRVD TIGQMSCNPS FGGIGKGHLM REVDALDGLC SRICDQSGVH YKVLNRRKGP
121AVWGLRAQID RKLYKQNMQK EILNTPLLTV QEGAVEDLIL TEPEPEHTGK CRVSGVVLVD
181GSTVYAESVI LTTGTFLRGM IVIGLETHPA GRLGDQPSIG LAQTLEKLGF VVGRLKTGTP
241PRIAKESINF SILNKHIPDN PSIPFSFTNE TVWIKPEDQL PCYLTHTNPR VDEIVLKNLH
301LNSHVKETTR GPRYCPSIES KVLRFPNRLH QVWLEPEGMD SDLIYPQGLS MTLPAELQEK
361MITCIRGLEK AKVIQPGYGV QYDYLDPRQI TPSLETHLVQ RLFFAGQING TTGYEEAAAQ
421GVIAGINASL RVSRKPPFVV SRTEGYIGVL IDDLTTLGTS EPYRMFTSRV EFRLSLRPDN
481ADSRLTLRGY KDAGCVSQQR YERACWMKSS LEEGISVLKS IEFLSSKWKK LIPEASISTS
541RSLPVRALDV LKYEEVDMDS LAKAVPEPLK KYTKCRELAE RLKIEATYES VLFHQLQEIK
601GVQQDEALQL PKDLDYLTIR DVSLSHEVRE KLHFSRPQTI GAASRIPGVT PAAIINLLRF
661VKTTQRRQSA MNESSKTDQY LCDADRLQER EL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

692 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.