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MTMR8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MTMR8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112299-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MTMR8 (NM_017677), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MTMR8 | Myotubularin-related protein 8 | Myotubularin related protein 8
Origin Species
Human
NCBI GenBank
NM_017677
SwissProt
Q96EF0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDHITVPKVE NVKLVDRYVS KKPANGILYL TATHLIYVEA SGAARKETWI ALHHIATVEK
61LPITSLGCPL TLRCKNFRVA HFVLDSDLVC HEVYISLLKL SQPALPEDLY AFSYNPKSSK
121EMRESGWKLI DPISDFGRMG IPNRNWTITD ANRNYEICST YPPEIVVPKS VTLGTVVGSS
181KFRSKERVPV LSYLYKENNA AICRCSQPLS GFYTRCVDDE LLLEAISQTN PGSQFMYVVD
241TRPKLNAMAN RAAGKGYENE DNYANIRFRF MGIENIHVMR SSLQKLLEVC ELKTPTMSEF
301LSGLESSGWL RHIKAIMDAG IFITKAVKVE KASVLVHCSD GWDRTAQVCS VASILLDPFY
361RTFKGLMILI EKEWISMGHK FSQRCGHLDG DSKEVSPIFT QFLDCIWQLM EQFPCAFEFN
421ENFLLEIHDH VFSCQFGNFL GNCQKDREDL RVYEKTHSVW PFLVQRKPDF RNPLYKGFTM
481YGVLNPSTVP YNIQFWCGMY NRFDKGLQPK QSMLESLLEI KKQRAMLETD VHELEKKLKV
541RDEPPEEICT CSQLGNILSQ HLGSPLTNPL GFMGINGDLN TLMENGTLSR EGGLRAQMDQ
601VKSQGADLHH NCCEIVGSLR AINISGDVGI SEAMGISGDM CTFEATGFSK DLGICGAMDI
661SEATGISGNL GISEARGFSG DMGILGDTGI SKASTKEADY SKHQTRTRPL EQKLISEEDL
721AANDILDYKD DDDKV

735 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.