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MTMR7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MTMR7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G116472-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MTMR7 (NM_004686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MTMR7 | Myotubularin-related protein 7 | Myotubularin related protein 7
Origin Species
Human
NCBI GenBank
AF073482
SwissProt
Q9Y216

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEHIRTPKVE NVRLVDRVSP KKAALGTLYL TATHVIFVEN SPDARKETWI LHSQISTIEK
61QATTATGCPL LIRCKNFQII QLIIPQERDC HDVYISLIRL ARPVKYEELY CFSFNPMLDK
121EEREQGWVLI DLSEEYTRMG LPNHYWQLSD VNRDYRVCDS YPTELYVPKS ATAHIIVGSS
181KFRSRRRFPV LSYYYKDNHA SICRSSQPLS GFSARCLEDE QMLQAIRKAN PGSDFVYVVD
241TRPKLNAMAN RAAGKGYENE DNYSNIKFQF IGIENIHVMR NSLQKMLEVC ELKSPSMSDF
301LWGLENSGWL RHIKAIMDAG IFIAKAVSEE GASVLVHCSD GWDRTAQVCS VASLLLDPHY
361RTLKGFMVLI EKDWISFGHK FNHRYGNLDG DPKEISPVID QFIECVWQLM EQFPCAFEFN
421ERFLIHIQHH IYSCQFGNFL CNSQKERREL KIQERTYSLW AHLWKNRADY LNPLFRADHS
481QTQGTLHLPT TPCNFMYKFW SGMYNRFEKG MQPRQSVTDY LMAVKEETQQ LEEELEALEE
541RLEKIQKVQL NCTKVKSKQS EPSKHSGFST SDNSIANTPQ DYSGNMKSFP SRSPSQGDED
601SALILTQDNL KSSDPDLSAN SDQESGVEDL SCRSPSGGEH APSEDSGKDR DSDEAVFLTA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

660 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.