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MTMR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MTMR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117057-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MTMR1 (NM_003828), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MTM-related phosphatase 1 | Myotubularin related protein 1 | MTMR1 | Myotubularin-related protein 1
Origin Species
Human
NCBI GenBank
NM_003828
SwissProt
Q13613

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRPAAAAAA GCEGGGGPNP GPAGGRRPPR AAGGATAGSR QPSVETLDSP TGSHVEWCKQ
61LIAATISSQI SGSVTSENVS RDYKALRDGN KLAQMEEAPL FPGESIKAIV KDVMYICPFM
121GAVSGTLTVT DFKLYFKNVE RDPHFILDVP LGVISRVEKI GAQSHGDNSC GIEIVCKDMR
181NLRLAYKQEE QSKLGIFENL NKHAFPLSNG QALFAFSYKE KFPINGWKVY DPVSEYKRQG
241LPNESWKISK INSNYEFCDT YPAIIVVPTS VKDDDLSKVA AFRAKGRVPV LSWIHPESQA
301TITRCSQPLV GPNDKRCKED EKYLQTIMDA NAQSHKLIIF DARQNSVADT NKTKGGGYES
361ESAYPNAELV FLEIHNIHVM RESLRKLKEI VYPSIDEARW LSNVDGTHWL EYIRMLLAGA
421VRIADKIESG KTSVVVHCSD GWDRTAQLTS LAMLMLDSYY RTIKGFETLV EKEWISFGHR
481FALRVGHGND NHADADRSPI FLQFVDCVWQ MTRQFPSAFE FNELFLITIL DHLYSCLFGT
541FLCNCEQQRF KEDVYTKTIS LWSYINSQLD EFSNPFFVNY ENHVLYPVAS LSHLELWVNY
601YVRWNPRMRP QMPIHQNLKE LLAVRAELQK RVEGLQREVA TRAVSSSSER GSSPSHSATS
661VHTSV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

665 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.