Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MTL5 / TESMIN Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116313-100
Catalog Number: LS-G116313
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MLT5(TESMIN) (NM_004923), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CXC domain containing 2 | CXCDC2 | MTL5 | MTLT | Tesmin
- Origin Species
- Human
- NCBI GenBank
- NM_004923
- SwissProt
- Q9Y4I5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEEGPLPGGL PSPEDAMVTE LLSPEGPFAS ENIGLKAPVK YEEDEFHVFK EAYLGPADPK61EPVLHAFNPA LGADCKGQVK AKLAGGDSDG GELLGEYPGI PELSALEDVA LLQAPQPPAC121NVHFLSSLLP AHRSPAVLPL GAWVLEGASH PGVRMIPVEI KEAGGTTTSN NPEEATLQNL181LAQESCCKFP SSQELEDASC CSLKKDSNPM VICQLKGGTQ MLCIDNSRTR ELKALHLVPQ241YQDQNNYLQS DVPKPMTALV GRFLPASTKL NLITQQLEGA LPSVVNGSAF PSGSTLPGPP301KITLAGYCDC FASGDFCNNC NCNNCCNNLH HDIERFKAIK ACLGRNPEAF QPKIGKGQLG361NVKPQHNKGC NCRRSGCLKN YCECYEAQIM CSSICKCIGC KNYEESPERK TLMSMPNYMQ421TGGLEGSHYL PPTKFSGLPR FSHDRRPSSC ISWEVVEATC ACLLAQGEEA EKEHCSKCLA481EQMILEEFGR CLSQILHTEF KSKGLKMESGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
508 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.