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MTIF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MTIF2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MTIF2 antibodies →
$725.00each
Catalog Number: LS-G122201-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IF 2(Mt)(MTIF2) (NM_001005369), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IF-2(Mt) | IF-2mt | MTIF2 | IF2(mt)
Origin Species
Human
NCBI GenBank
NM_002453
SwissProt
P46199

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.34 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNQKLLKLEN LLRFHTIYRQ LHSLCQRRAL RQWRHGFSSA YPVWTAQLCA WPWPTDVLNG
61AALSQYRLLV TKKEEGPWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT
121DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKVR KNKDAVRRPQ ADPALLTPRS
181PVVTIMGHVD HGKTTLLDKF RKTQVAAVET GGITQHIGAF LVSLPSGEKI TFLDTPGHAA
241FSAMRARGAQ VTDIVVLVVA ADDGVMKQTV ESIQHAKDAQ VPIILAVNKC DKAEADPEKV
301KKELLAYDVV CEDYGGDVQA VPVSALTGDN LMALAEATVA LAEMLELKAD PNGPVEGTVI
361ESFTDKGRGL VTTAIIQRGT LRKGSVLVAG KCWAKVRLMF DENGKTIDEA YPSMPVGITG
421WRDLPSAGEE ILEVESEPRA REVVDWRKYE QEQEKGQEDL KIIEEKRKEH KEAHQKAREK
481YGHLLWKKRS ILRFLERKEQ IPLKPKEKRE RDSNVLSVII KGDVDGSVEA ILNIIDTYDA
541SHECELELVH FGVGDISAND VNLAETFDGV IYGFNVNAGN VIQQSAAKKG VKIKLHKIIY
601RLVEDLQEEL SSRLPCAVEE HPVGEASILA TFSVTEGKKK VPVAGCRVQK GQLEKQKKFK
661LTRNGHVIWK GSLTSLKHHK DDISIVKTGM DCGLSLDEDN MEFQVGDRIV CYEEKQIQAK
721TSWDPGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

727 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.