Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MTG2 / GTPBP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113027-100
Catalog Number: LS-G113027
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GTPBP5(MTG2) (NM_015666), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ1005F21.2 | GTP-binding protein 5 | GTPBP5 | Protein obg homolog 1 | ObgH1
- Origin Species
- Human
- NCBI GenBank
- AL117498
- SwissProt
- Q9H4K7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPARCFSAR LRTVFQGVGH WALSTWAGLK PSRLLPQRAS PRLLSVGRAD LAKHQELPGK61KLLSEKKLKR YFVDYRRVLV CGGNGGAGAS CFHSEPRKEF GGPDGGDGGN GGHVILRVDQ121QVKSLSSVLS RYQGFSGEDG GSKNCFGRSG AVLYIRVPVG TLVKEGGRVV ADLSCVGDEY181IAALGGAGGK GNRFFLANNN RAPVTCTPGQ PGQQRVLHLE LKTVAHAGMV GFPNAGKSSL241LRAISNARPA VASYPFTTLK PHVGIVHYEG HLQIAVADIP GIIRGAHQNR GLGSAFLRHI301ERCRFLLFVV DLSQPEPWTQ VDDLKYELEM YEKGLSARPH AIVANKIDLP EAQANLSQLR361DHLGQEVIVL SALTGENLEQ LLLHLKVLYD AYAEAELGQG RQPLRWTRTRPLEQKLISEEDLAANDILDYKDDDDKV
406 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.