Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MTERF4 / MTERFD2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104285-100
Catalog Number: LS-G104285
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MTERFD2(MTERF4) (NM_182501), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MTERFD2 | MTERF domain containing 2
- Origin Species
- Human
- SwissProt
- Q8BVN4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 43.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAFGRQVLD WHRLIPLTWA CMARQTPHLG EQRRTTASLL RKLTTASNGG VIEELSCVRS61NNYVQEPECR RNLVQCLLEK QGTPVVQGSL ELERVMSSLL DMGFSNAHIN ELLSVRRGAS121LQQLLDIISE FILLGLNPEP VCVVLKKSPQ LLKLPIMQMR KRSSYLQKLG LGEGKLKRVL181YCCPEIFTMR QQDINDTVRL LKEKCLFTVQ QVTKILHSCP SVLREDLGQL EYKFQYAYFR241MGIKHPDIVK SEYLQYSLTK IKQRHIYLER LGRYQTPDKK GQTQIPNPLL KDILRVSEAE301FLARTARTSV EEFQVFKKLL AREEEESESS TSDDKRASLD EDEDDDDEED NDEDDNDEDD361DDEDDDEAED NDEDEDDDEE ETRTRPLEQKLISEEDLAANDILDYKDDDDKV
381 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.