Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MSTO1 / MST Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111978-100
Catalog Number: LS-G111978
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MSTO1 (NM_018116), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LST005 | Protein misato homolog 1 | Misato homolog 1 (Drosophila) | MST | MSTO1
- Origin Species
- Human
- NCBI GenBank
- NM_018116
- SwissProt
- Q9BUK6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGGAREVLT LQLGHFAGFV GAHWWNQQDA ALGRATDSKE PPGELCPDVL YRTGRTLHGQ61ETYTPRLILM DLKGSLSSLK EEGGLYRDKQ LDAAIAWQGK LTTHKEELYP KNPYLQDFLS121AEGVLSSDGV WRVKSIPNGK GSSPLPTATT PKPLIPTEAS IRVWSDFLRV HLHPRSICMI181QKYNHDGEAG RLEAFGQGES VLKEPKYQEE LEDRLHFYVE ECDYLQGFQI LCDLHDGFSG241VGAKAAELLQ DEYSGRGIIT WGLLPGPYHR GEAQRNIYRL LNTAFGLVHL TAHSSLVCPL301SLGGSLGLRP EPPVSFPYLH YDATLPFHCS AILATALDTV TVPYRLCSSP VSMVHLADML361SFCGKKVVTA GAIIPFPLAP GQSLPDSLMQ FGGATPWTPL SACGEPSGTR CFAQSVVLRG421IDRACHTSQL TPGTPPPSAL HACTTGEEIL AQYLQQQQPG VMSSSHLLLT PCRVAPPYPH481LFSSCSPPGM VLDGSPKGAA VESIPVFGAL CSSSSLHQTL EALARDLTKL DLRRWASFMD541AGVEHDDVAE LLQELQSLAQ CYQGGDSLVDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
570 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.