Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MST1R / RON Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117975-100
Catalog Number: LS-G117975
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RON(MST1R) (NM_002447), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-met-related tyrosine kinase | CD136 | CDw136 | CD136 antigen | Friend virus susceptibility 2 | Fv2 | MSP receptor | p185-Ron | PTK8 protein tyrosine kinase 8 | RON | Soluble RON variant 3 | Soluble RON variant 1 | MST1R | MST1R variant RON30 | Oncogene RON | PTK8 | MST1R variant RON62 | Protein-tyrosine kinase 8 | RON variant E2E3 | Soluble RON variant 2 | Soluble RON variant 4
- Origin Species
- Human
- NCBI GenBank
- NM_002447
- SwissProt
- Q04912
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 152.37 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELLPPLPQS FLLLLLLPAK PAAGEDWQCP RTPYAASRDF DVKYVVPSFS AGGLVQAMVT61YEGDRNESAV FVAIRNRLHV LGPDLKSVQS LATGPAGDPG CQTCAACGPG PHGPPGDTDT121KVLVLDPALP ALVSCGSSLQ GRCFLHDLEP QGTAVHLAAP ACLFSAHHNR PDDCPDCVAS181PLGTRVTVVE QGQASYFYVA SSLDAAVAAS FSPRSVSIRR LKADASGFAP GFVALSVLPK241HLVSYSIEYV HSFHTGAFVY FLTVQPASVT DDPSALHTRL ARLSATEPEL GDYRELVLDC301RFAPKRRRRG APEGGQPYPV LRVAHSAPVG AQLATELSIA EGQEVLFGVF VTGKDGGPGV361GPNSVVCAFP IDLLDTLIDE GVERCCESPV HPGLRRGLDF FQSPSFCPNP PGLEALSPNT421SCRHFPLLVS SSFSRVDLFN GLLGPVQVTA LYVTRLDNVT VAHMGTMDGR ILQVELVRSL481NYLLYVSNFS LGDSGQPVQR DVSRLGDHLL FASGDQVFQV PIQGPGCRHF LTCGRCLRAW541HFMGCGWCGN MCGQQKECPG SWQQDHCPPK LTEFHPHSGP LRGSTRLTLC GSNFYLHPSG601LVPEGTHQVT VGQSPCRPLP KDSSKLRPVP RKDFVEEFEC ELEPLGTQAV GPTNVSLTVT661NMPPGKHFRV DGTSVLRGFS FMEPVLIAVQ PLFGPRAGGT CLTLEGQSLS VGTSRAVLVN721GTECLLARVS EGQLLCATPP GATVASVPLS LQVGGAQVPG SWTFQYREDP VVLSISPNCG781YINSHITICG QHLTSAWHLV LSFHDGLRAV ESRQCERQLP EQQLCRLPEY VVRDPQGWVA841GNLSARGDGA AGFTLPGFRF LPPPHPPSAN LVPLKPEEHA IKFEYIGLGA VADCVGINVT901VGGESCQHEF RGDMVVCPLP PSLQLGQDGA PLQVCVDGEC HILGRVVRPG PDGVPQSTLL961GILLPLLLLV AALATALVFS YWWRRKQLVL PPNLNDLASL DQTAGATPLP ILYSGSDYRS1021GLALPAIDGL DSTTCVHGAS FSDSEDESCV PLLRKESIQL RDLDSALLAE VKDVLIPHER1081VVTHSDRVIG KGHFGVVYHG EYIDQAQNRI QCAIKSLSRI TEMQQVEAFL REGLLMRGLN1141HPNVLALIGI MLPPEGLPHV LLPYMCHGDL LQFIRSPQRN PTVKDLISFG LQVARGMEYL1201AEQKFVHRDL AARNCMLDES FTVKVADFGL ARDILDREYY SVQQHRHARL PVKWMALESL1261QTYRFTTKSD VWSFGVLLWE LLTRGAPPYR HIDPFDLTHF LAQGRRLPQP EYCPDSLYQV1321MQQCWEADPA VRPTFRVLVG EVEQIVSALL GDHYVQLPAT YMNLGPSTSH EMNVRPEQPQ1381FSPMPGNVRR PRPLSEPPRP TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1401 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.