Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MSANTD2 / C11orf61 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109801-100
Catalog Number: LS-G109801
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MSANTD2 (NM_024631), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C11orf61 | MSANTD2
- Origin Species
- Human
- NCBI GenBank
- NM_024631
- SwissProt
- Q6P1R3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPCGSELP ANSPLKIPKM EVLSPASPGG LSDGNPSLSD PSTPRGASPL GPGSAAGSGA61AASGGLGLGL GGRSAASSSV SFSPGGGGGG AAAAAAAACR GMSWTPAETN ALIAVWGNER121LVEARYQQLE GAGTVFGSKA PGPAMYERVS RALAELGYER TPSQCRERIK ELESDGSTME181DYSQEDWGNH SQDLHGYPTD QELDEIPVTK RTLKIKQESS EEAQKRDIMQ NIVQILESVQ241LKWELFQSWT DFSRLHLSNK LAIFGIGYNT RWKEDIRYHY AEISSQVPLG KRLREYFNSE301KPEGRIIMTR VQKMNWKNVY YKFLEITISE ARCLELHMEI DWIPIAHSKP TGGNVVQYLL361PGGIPKSPGL YAIGYEECIE RPLSPHMEQS SLDPGKEGRV DLETLSAQAS LQVEIEPTRI421IYCYLGIAEV RTLQQCLFLH FQANTKTFSK DWVGINGFLS QNCIVDPGVS PKSIYIKFVE481VERDFLSAGS LVECLEKAIG YPLKFNNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
507 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.