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MRVI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MRVI1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MRVI1 antibodies →
$725.00each
Catalog Number: LS-G119266-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MRVI1 (NM_001098579), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
IRAG | MRVI1 | JAW1-related protein MRVI1 | JAW1L | Protein MRVI1
Origin Species
Human
NCBI GenBank
NM_006069
SwissProt
Q9Y6F6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGMDLTCPFG VSPACGAQAS WSIFGADAAE VPGTRGHSQQ EAAMPHIPED EEPPGEPQAA
61QSPAGQGPPT AGVSCSPTPT IVLTGDATSP EGETDKNLAN RVHSPHKRLS HRHLKVSTAS
121LTSVDPAGHI IDLVNDQLPD ISISEEDKKK NLALLEEAKL VSERFLTRRG RKSRSSPGDS
181PSAVSPNLSP SASPTSSRSN SLTVPTPPGL DVCSGPPSPL PGAPPQKGDE ADVSSPHPGE
241PNVPKGLADR KQNDQRKVSQ GRLAPRPPPV EKSKEIAIEQ KENFDPLQYP ETTPKGLAPV
301TNSSGKMALN SPQPGPVESE LGKQLLKTGW EGSPLPRSPT QDAAGVGPPA SQGRGPAGEP
361MGPEAGSKAE LPPTVSRPPL LRGLSWDSGP EEPGPRLQKV LAKLPLAEEE KRFAGKAGGK
421LAKAPGLKDF QIQVQPVRMQ KLTKLREEHI LMRNQNLVGL KLPDLSEAAE QEKGLPSELS
481PAIEEEESKS GLDVMPNISD VLLRKLRVHR SLPGSAPPLT EKEVENVFVQ LSLAFRNDSY
541TLESRINQAE RERNLTEENT EKELENFKAS ITSSASLWHH CEHRETYQKL LEDIAVLHRL
601AARLSSRAEV VGAVRQEKRM SKATEVMMQY VENLKRTYEK DHAELMEFKK LANQNSSRSC
661GPSEDGVPRT ARSMSLTLGK NMPRRRVSVA VVPKFNALNL PGQTPSSSSI PSLPALSESP
721NGKGSLPVTS ALPALLENGK TNGDPDCEAS APALTLSCLE ELSQETKARM EEEAYSKGFQ
781EGLKKTKELQ DLKEEEEEQK SESPEEPEEV EETEEEEKGP RSSKLEELVH FLQVMYPKLC
841QHWQVIWMMA AVMLVLTVVL GLYNSYNSCA EQADGPLGRS TCSAAQRDSW WSSGLQHEQP
901TEQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

903 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.