Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MROH1 / HEATR7A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119052-100
Catalog Number: LS-G119052
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HEATR7A(MROH1) (NM_001099281), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTESSMKKLA STLLDAITDK DPLVQEQVCS ALCSLGEVRP VETLRACEEY LRQHDKLAHP61YRAAVLRAME RVLSSRASEL DKDTASTIIL LASSEMTKTK DLVWDWQQAA SGVLVAVGRQ121FISKVMEELL RRLHPGTLPH CAVLHTLASL SVANAFGVVP FLPSVLSSLL PVLGVAKQDT181VRVAFCSALQ RFSEGALEYL ANLDRAPDPT VRKDAFATDI FSAYDVLFHQ WLQSREAKLR241LAVVEALGPM SHLLPSERLE EQLPKLLPGI LALYKKHAET FYLSKSLGQI LEAAVSVGSR301TLETQLDALL AALHSQICVP VESSSPLVMS NQKEVLRCFT VLACSSPDRL LAFLLPRLDT361SNERTRVGTL QVVRHVINSA GSTNTRITLM LPSAVTRLKH EDCPARAAPQ PADLTAAPAS421VASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
422 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.