Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MRNP41 / RAE1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121644-100
Catalog Number: LS-G121644
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RAE1 (NM_001015885), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ481F12.3 | DJ800J21.1 | Mnrp41 | MRNA export factor | MRNA-binding protein | 41-kD | Rae1 protein homolog | MIG14 | Migration-inducing gene 14 | MRNA export protein | MRNP41 | RAE1
- Origin Species
- Human
- NCBI GenBank
- NM_003610
- SwissProt
- P78406
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLFGTTSGF GTSGTSMFGS ATTDNHNPMK DIEVTSSPDD SIGCLSFSPP TLPGNFLIAG61SWANDVRCWE VQDSGQTIPK AQQMHTGPVL DVCWSDDGSK VFTASCDKTA KMWDLSSNQA121IQIAQHDAPV KTIHWIKAPN YSCVMTGSWD KTLKFWDTRS SNPMMVLQLP ERCYCADVIY181PMAVVATAER GLIVYQLENQ PSEFRRIESP LKHQHRCVAI FKDKQNKPTG FALGSIEGRV241AIHYINPPNP AKDNFTFKCH RSNGTNTSAP QDIYAVNGIA FHPVHGTLAT VGSDGRFSFW301DKDARTKLKT SEQLDQPISA CCFNHNGNIF AYASSYDWSK GHEFYNPQKK NYIFLRNAAE361ELKPRNKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
368 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.