Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MRGPRX4 / MRGX4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102179-100
Catalog Number: LS-G102179
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MRGPRX4 (NM_054032), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MRGX4 | MRGPRX4 | SNSR6 | SNSR5 | MAS-related GPR | member X4
- Origin Species
- Human
- NCBI GenBank
- NM_054032
- SwissProt
- Q96LA9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPTVPVFGT KLTPINGREE TPCYNQTLSF TVLTCIISLV GLTGNAVVLW LLGYRMRRNA61VSIYILNLAA ADFLFLSFQI IRSPLRLINI SHLIRKILVS VMTFPYFTGL SMLSAISTER121CLSVLWPIWY RCRRPTHLSA VVCVLLWGLS LLFSMLEWRF CDFLFSGADS SWCETSDFIP181VAWLIFLCVV LCVSSLVLLV RILCGSRKMP LTRLYVTILL TVLVFLLCGL PFGILGALIY241RMHLNLEVLY CHVYLVCMSL SSLNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDKPE301VDKGEGQLPE ESLELSGSRL GPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
322 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.