Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MRGPRX3 / MRGX3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102177-100
Catalog Number: LS-G102177
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MRGX3(MRGPRX3) (NM_054031), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAS-related GPR | member X3 | Hdrr1 | HDRR2 | MRGX3 | SNSR1 | SNSR2 | Mas-related GPCR member X3 | MRGPRX3
- Origin Species
- Human
- NCBI GenBank
- NM_054031
- SwissProt
- Q96LB0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDSTIPVLGT ELTPINGREE TPCYKQTLSF TGLTCIVSLV ALTGNAVVLW LLGCRMRRNA61VSIYILNLVA ADFLFLSGHI IRSPLRLINI RHPISKILSP VMTFPYFIGL SMLSAISTER121CLSILWPIWY HCRRPRYLSS VMCVLLWALS LLRSILEWMF CDFLFSGADS VWCETSDFIT181IAWLVFLCVV LCGSSLVLLV RILCGSRKMP LTRLYVTILL TVLVFLLCGL PFGIQWALFS241RIHLDWKVLF CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDTPE301VDEGGGQLPQ ETLELSGSRL EQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
322 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.