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MPP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MPP7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MPP7 antibodies →
$504.00each
Catalog Number: LS-G105311-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MPP7 (NM_173496), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MAGUK p55 subfamily member 7 | MPP7 | RP11-218D6.5
Origin Species
Human
SwissProt
Q5T2T1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MPALSTGSGS DTGLYELLAA LPAQLQPHVD SQEDLTFLWD MFGEKSLHSL VKIHEKLHYY
61EKQSPVPILH GAAALADDLA EELQNKPLNS EIRELLKLLS KPNVKALLSV HDTVAQKNYD
121PVLPPMPEDI DDEEDSVKII RLVKNREPLG ATIKKDEQTG AIIVARIMRG GAADRSGLIH
181VGDELREVNG IPVEDKRPEE IIQILAQSQG AITFKIIPGS KEETPSKEGK MFIKALFDYN
241PNEDKAIPCK EAGLSFKKGD ILQIMSQDDA TWWQAKHEAD ANPRAGLIPS KHFQERRLAL
301RRPEILVQPL KVSNRKSSGF RRSFRLSRKD KKTNKSMYEC KKSDQYDTAD VPTYEEVTPY
361RRQTNEKYRL VVLVGPVGVG LNELKRKLLI SDTQHYGVTV PHTTRARRSQ ESDGVEYIFI
421SKHLFETDVQ NNKFIEYGEY KNNYYGTSID SVRSVLAKNK VCLLDVQPHT VKHLRTLEFK
481PYVIFIKPPS IERLRETRKN AKIISSRDDQ GAAKPFTEED FQEMIKSAQI MESQYGHLFD
541KIIINDDLTV AFNELKTTFD KLETETHWVP VSWLHS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

576 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.