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MPP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MPP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G118288-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MPP3 (NM_001932), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Discs large homolog 3 | Discs | large homolog 3 | Protein MPP3 | MAGUK p55 subfamily member 3 | MPP3
Origin Species
Human
NCBI GenBank
NM_001932
SwissProt
Q13368

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPVLSEDSGL HETLALLTSQ LRPDSNHKEE MGFLRDVFSE KSLSYLMKIH EKLRYYERQS
61PTPVLHSAVA LAEDVMEELQ AASVHSDERE LLQLLSTPHL RAVLMVHDTV AQKNFDPVLP
121PLPDNIDEDF DEESVKIVRL VKNKEPLGAT IRRDEHSGAV VVARIMRGGA ADRSGLVHVG
181DELREVNGIA VLHKRPDEIS QILAQSQGSI TLKIIPATQE EDRLKESKVF MRALFHYNPR
241EDRAIPCQEA GLPFQRRQVL EVVSQDDPTW WQAKRVGDTN LRAGLIPSKG FQERRLSYRR
301AAGTLPSPQS LRKPPYDQPC DKETCDCEGY LKGHYVAGLR RSFRLGCRER LGGSQEGKMS
361SGAESPELLT YEEVARYQHQ PGERPRLVVL IGSLGARLHE LKQKVVAENP QHFGVAVPHT
421TRPRKSHEKE GVEYHFVSKQ AFEADLHHNK FLEHGEYKEN LYGTSLEAIQ AVMAKNKVCL
481VDVEPEALKQ LRTSEFKPYI IFVKPAIQEK RKTPPMSPAC EDTAAPFDEQ QQEMAASAAF
541IDRHYGHLVD AVLVKEDLQG AYSQLKVVLE KLSKDTHWVP VSWVR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

585 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.