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MPHOSPH9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MPHOSPH9 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MPHOSPH9 antibodies →
$725.00each
Catalog Number: LS-G110258-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MPHOSPH9 (NM_022782), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
M-phase phosphoprotein 9 | MPP9 | MPP-9 | MPHOSPH9
Origin Species
Human
NCBI GenBank
NM_022782
SwissProt
Q99550

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
116 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGFFSLSSER NESVIHYPES TEPEIQQEMS TSQPDCNVDS CSVSSGYGTF CISELNLYKS
61KDPKEFMEHI DVPKGQYVAP AVPAESLVDG VKNENFYIQT PEECHVSLKE DVSISPGEFE
121HNFLGENKVS EVYSGKTNSN AITSWAQKLK QNQPKRAHVE DGGSRSKQGN EQSKKTPIEK
181SDFAAATHPR AFYLSKPDET PNAWMSDSGT GLTYWKLEEK DMHHSLPETL EKTFISLSST
241DVSPNQSNTS NEMKLPSLKD IYYKKQRENK QLPERNLTSA SNPNHPPEVL TLDPTLHMKP
301KQQISGIQPH GLPNALDDRI SFSPDSVLEP SMSSPSDIDS FSQASNVTSQ LPGFPKYPSH
361TKASPVDSWK NQTFQNESRT SSTFPSVYTI TSNDISVNTV DEENTVMVAS ASVSQSQLPG
421TANSVPECIS LTSLEDPVIL SKIRQNLKEK HARHIADLRA YYESEINSLK QKLEAKEISG
481VEDWKITNQI LVDRCGQLDS ALHEATSRVR TLENKNNLLE IEVNDLRERF SAASSASKIL
541QERIEEMRTS SKEKDNTIIR LKSRLQDLEE AFENAYKLSD DKEAQLKQEN KMFQDLLGEY
601ESLGKEHRRV KDALNTTENK LLDAYTQISD LKRMISKLEA QVKQVEHENM LSLRHNSRIH
661VRPSRANTLA TSDVSRRKWL IPGAEYSIFT GQPLDTQDSN VDNQLEETCS LGHRSPLEKD
721SSPGSSSTSL LIKKQRETSD TPIMRALKEL DEGKIFKNWG TQTEKEDTSN INPRQTETSV
781NASRSPEKCA QQRQKRLNSA SQRSSSLPPS NRKSSTPTKR EIMLTPVTVA YSPKRSPKEN
841LSPGFSHLLS KNESSPIRFD ILLDDLDTVP VSTLQRTNPR KQLQFLPLDD SEEKTYSEKA
901TDNHVNHSSC PEPVPNGVKK VSVRTAWEKN KSVSYEQCKP VSVTPQGNDF EYTAKIRTLA
961ETERFFDELT KEKDQIEAAL SRMPSPGGRI TLQTRLNQEA LEDRLERINR ELGSVRMTLK
1021KFHVLRTSAN L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1031 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.