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MPEG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MPEG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MPEG1 antibodies →
$725.00each
Catalog Number: LS-G120596-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MPEG1 (NM_001039396), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Macrophage expressed gene 1 | Mpg-1 | MPS1 | Macrophage expressed 1 | Macrophage gene 1 protein | MPEG1 | MPG1
Origin Species
Human
NCBI GenBank
NM_001039396
SwissProt
Q2M385

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNNFRATILF WAAAAWAKSG KPSGEMDEVG VQKCKNALKL PVLEVLPGGG WDNLRNVDMG
61RVMELTYSNC RTTEDGQYII PDEIFTIPQK QSNLEMNSEI LESWANYQSS TSYSINTELS
121LFSKVNGKFS TEFQRMKTLQ VKDQAITTRV QVRNLVYTVK INPTLELSSG FRKELLDISD
181RLENNQTRMA TYLAELLVLN YGTHVTTSVD AGAALIQEDH LRASFLQDSQ SSRSAVTASA
241GLAFQNTVNF KFEENYTSQN VLTKSYLSNR TNSRVQSIGG VPFYPGITLQ AWQQGITNHL
301VAIDRSGLPL HFFINPNMLP DLPGPLVKKV SKTVETAVKR YYTFNTYPGC TDLNSPNFNF
361QANTDDGSCE GKMTNFSFGG VYQECTQLSG NRDVLLCQKL EQKNPLTGDF SCPSGYSPVH
421LLSQIHEEGY NHLECHRKCT LLVFCKTVCE DVFQVAKAEF RAFWCVASSQ VPENSGLLFG
481GLFSSKSINP MTNAQSCPAG YFPLRLFENL KVCVSQDYEL GSRFAVPFGG FFSCTVGNPL
541VDPAISRDLG ALSLKKCPGG FSQHPALISD GCQVSYCVKS GLFTGGSLPP ARLPPFTRPP
601LMSQAATNTV IVTNSENARS WIKDSQTHQW RLGEPIELRR AMNVIHGDGG GLSGGAAAGV
661TVGVTTILAV VITLAIYGTR KFKKKAYQAI EERQSLVPGT AATGDTTYQE QGQSPA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

716 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.