Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MOX / MOXD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113169-100
Catalog Number: LS-G113169
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MOXD1 (NM_015529), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DKFZP564G202 | DJ248E1.1 | Monooxygenase | DBH-like 1 | Monooxygenase X | MOX | MOXD1 | PRO5780
- Origin Species
- Human
- NCBI GenBank
- W45115
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCCWPLLLLW GLLPGTAAGG SGRTYPHRTL LDSEGKYWLG WSQRGSQIAF RLQVRTAGYV61GFGFSPTGAM ASADIVVGGV AHGRPYLQDY FTNANRELKK DAQQDYHLEY AMENSTHTII121EFTRELHTCD INDKSITDST VRVIWAYHHE DAGEAGPKYH DSNRGTKSLR LLNPEKTSVL181STALPYFDLV NQDVPIPNKD TTYWCQMFKI PVFQEKHHVI KVEPVIQRGH ESLVHHILLY241QCSNNFNDSV LESGHECYHP NMPDAFLTCE TVIFAWAIGG EGFSYPPHVG LSLGTPLDPH301YVLLEVHYDN PTYEEGLIDN SGLRLFYTMD IRKYDAGVIE AGLWVSLFHT IPPGMPEFQS361EGHCTLECLE EALEAEKPSG IHVFAVLLHA HLAGRGIRLR HFRKGKEMKL LAYDDDFDFN421FQEFQYLKEE QTILPGDNLI TECRYNTKDR AEMTWGGLST RSEMCLSYLL YYPRINLTRC481ASIPDIMEQL QFIGVKEIYR PVTTWPFIIK SPKQYKNLSF MDAMNKFKWT KKEGLSFNEL541VLSLPVNVRC SKTDNAEWSI QGMTALPPDI ERPYKAEPLV CGTSSSSSLH RDFSINLLVC601LLLLSCTLST KSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
613 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.