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MOV10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MOV10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MOV10 antibodies →
$725.00each
Catalog Number: LS-G124992-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MOV10 (NM_001130079), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FSAP113 | Gb110 | MOV10 | KIAA1631 | Putative helicase MOV-10
Origin Species
Human
NCBI GenBank
NM_020963
SwissProt
Q9HCE1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSKFSCRQL REAGQCFESF LVVRGLDMET DRERLRTIYN RDFKISFGTP APGFSSMLYG
61MKIANLAYVT KTRVRFFRLD RWADVRFPEK RRMKLGSDIS KHHKSLLAKI FYDRAEYLHG
121KHGVDVEVQG PHEARDGQLL IRLDLNRKEV LTLRLRNGGT QSVTLTHLFP LCRTPQFAFY
181NEDQELPCPL GPGECYELHV HCKTSFVGYF PATVLWELLG PGESGSEGAG TFYIARFLAA
241VAHSPLAAQL KPMTPFKRTR ITGNPVVTNR IEEGERPDRA KGYDLELSMA LGTYYPPPRL
301RQLLPMLLQG TSIFTAPKEI AEIKAQLETA LKWRNYEVKL RLLLHLEELQ MEHDIRHYDL
361ESVPMTWDPV DQNPRLLTLE VPGVTESRPS VLRGDHLFAL LSSETHQEDP ITYKGFVHKV
421ELDRVKLSFS MSLLSRFVDG LTFKVNFTFN RQPLRVQHRA LELTGRWLLW PMLFPVAPRD
481VPLLPSDVKL KLYDRSLESN PEQLQAMRHI VTGTTRPAPY IIFGPPGTGK TVTLVEAIKQ
541VVKHLPKAHI LACAPSNSGA DLLCQRLRVH LPSSIYRLLA PSRDIRMVPE DIKPCCNWDA
601KKGEYVFPAK KKLQEYRVLI TTLITAGRLV SAQFPIDHFT HIFIDEAGHC MEPESLVAIA
661GLMEVKETGD PGGQLVLAGD PRQLGPVLRS PLTQKHGLGY SLLERLLTYN SLYKKGPDGY
721DPQFITKLLR NYRSHPTILD IPNQLYYEGE LQACADVVDR ERFCRWAGLP RQGFPIIFHG
781VMGKDEREGN SPSFFNPEEA ATVTSYLKLL LAPSSKKGKA RLSPRSVGVI SPYRKQVEKI
841RYCITKLDRE LRGLDDIKDL KVGSVEEFQG QERSVILIST VRSSQSFVQL DLDFNLGFLK
901NPKRFNVAVT RAKALLIIVG NPLLLGHDPD WKVFLEFCKE NGGYTGCPFP AKLDLQQGQN
961LLQGLSKLSP STSGPHSHDY LPQEREGEGG LSLQVEPEWR NEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1003 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.