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MON1B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MON1B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MON1B antibodies →
$504.00each
Catalog Number: LS-G113573-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MON1B (NM_014940), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSRG1 | MON1B | MON1 homolog B (yeast) | SAND2 | SRG1 | KIAA0872
Origin Species
Human
NCBI GenBank
NM_014940
SwissProt
Q7L1V2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVGGDTAAP APGGAEDLED TQFPSEEARE GGGVHAVPPD PEDEGLEETG SKDKDQPPSP
61SPPPQSEALS STSRLWSPAA PENSPTCSPE SSSGGQGGDP SDEEWRSQRK HVFVLSEAGK
121PIYSRYGSVE ALSATMGVMT ALVSFVQSAG DAIRAIYAED HKLVFLQQGP LLLVAMSRTS
181QSAAQLRGEL LAVHAQIVST LTRASVARIF AHKQNYDLRR LLAGSERTLD RLLDSMEQDP
241GALLLGAVRC VPLARPLRDA LGALLRRCTA PGLALSVLAV GGRLITAAQE RNVLAECRLD
301PADLQLLLDW VGAPAFAAGE AWAPVCLPRF NPDGFFYAYV ARLDAMPVCL LLLGTQREAF
361HAMAACRRLV EDGMHALGAM RALGEAASFS NASSASAPAY SVQAVGAPGL RHFLYKPLDI
421PDHHRQLPQF TSPELEAPYS REEERQRLSD LYHRLHARLH STSRPLRLIY HVAEKETLLA
481WVTSKFELYT CLSPLVTKAG AILVVTKLLR WVKKEEDRLF IRYPPKYSTP PATSTDQAAH
541NGLFTGL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

547 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.