Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MON1A / SAND1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108886-100
Catalog Number: LS-G108886
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MON1A (NM_032355), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MON1A | SAND1 | MON1 homolog A (yeast)
- Origin Species
- Human
- NCBI GenBank
- NM_032355
- SwissProt
- Q86VX9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATDMQRKRS SECLDGTLTP SDGQSMERAE SPTPGMAQGM EPGAGQEGAM FVHARSYEDL61TESEDGAASG DSHKEGTRGP PPLPTDMRQI SQDFSELSTQ LTGVARDLQE EMLPGSSEDW121LEPPGAVGRP ATEPPREGTT EGDEEDATEA WRLHQKHVFV LSEAGKPVYS RYGSEEALSS181TMGVMVALVS FLEADKNAIR SIHADGYKVV FVRRSPLVLV AVARTRQSAQ ELAQELLYIY241YQILSLLTGA QLSHIFQQKQ NYDLRRLLSG SERITDNLLQ LMARDPSFLM GAARCLPLAA301AVRDTVSASL QQARARSLVF SILLARNQLV ALVRRKDQFL HPIDLHLLFN LISSSSSFRE361GEAWTPVCLP KFNAAGFFHA HISYLEPDTD LCLLLVSTDR EDFFAVSDCR RRFQERLRKR421GAHLALREAL RTPYYSVAQV GIPDLRHFLY KSKSSGLFTS PEIEAPYTSE EEQERLLGLY481QYLHSRAHNA SRPLKTIYYT GPNENLLAWV TGAFELYMCY SPLGTKASAV SAIHKLMRWI541RKEEDRLFIL TPLTYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
555 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.