Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MOK / RAGE Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114082-100
Catalog Number: LS-G114082
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MOK protein kinase(MOK) (NM_014226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MOK | MOK kinase | MOK protein kinase | RAGE | RAGE1 | RAGE-1 | Renal tumor antigen 1 | Renal tumor antigen
- Origin Species
- Human
- NCBI GenBank
- NM_014226
- SwissProt
- Q9UQ07
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQRFE SIEQVNNLRE IQALRRLNPH61PNILMLHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRYPL SEKKIMHYMY QLCKSLDHIH121RNGIFHRDVK PENILIKQDV LKLGDFGSCR SVYSKQPYTE YISTRWYRAP ECLLTDGFYT181YKMDLWSAGC VFYEIASLQP LFPGVNELDQ ISKIHDVIGT PAQKILTKFK QSRAMNFDFP241FKKGSGIPLL TTNLSPQCLS LLHAMVAYDP DERIAAHQAL QHPYFQEQRK TEKRALGSHR301KAGFPEHPVA PEPLSNSCQI SKEGRKQKQS LKQEEDRPKR RGPAYVMELP KLKLSGVVRL361SSYSSPTLQS VLGSGTNGRV PVLRPLKCIP ASKKTDPQKD LKPAPQQCRL PTIVRKGGRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
419 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.