Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MOCOS / MoCo Sulfurase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112106-100
Catalog Number: LS-G112106
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MOCOS (NM_017947), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HMCS | MCS | MoCo sulfurase | MOCOS | Molybdenum cofactor sulfurase
- Origin Species
- Human
- NCBI GenBank
- NM_017947
- SwissProt
- Q96EN8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 97.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGAAAESGR ELWTFAGSRD PSAPRLAYGY GPGSLRELRA REFSRLAGTV YLDHAGATLF61SQSQLESFTS DLMENTYGNP HSQNISSKLT HDTVEQVRYR ILAHFHTTAE DYTVIFTAGS121TAALKLVAEA FPWVSQGPES SGSRFCYLTD SHTSVVGMRN VTMAINVISI PVRPEDLWSA181EERGASASNP DCQLPHLFCY PAQSNFSGVR YPLSWIEEVK SGRLRPVSTP GKWFVLLDAA241SYVSTSPLDL SAHQADFVPI SFYKIFGFPT GLGALLVHNR AAPLLRKTYF GGGTASAYLA301GEDFYIPRQS VAQRFEDGTI SFLDVIALKH GFDTLERLTG GMENIKQHTF TLAQYTYMAL361SSLQYPNGAP VVRIYSDSEF SSPEVQGPII NFNVLDDKGN IIGYSQVDKM ASLYNIHLRT421GCFCNTGACQ RHLGISNEMV RKHFQAGHVC GDNMDLIDGQ PTGSVRISFG YMSTLDDVQA481FLRFIIDTRL HSSGDWPVPQ AHADTGETGA PSADSQADVI PAVMGRRSLS PQEDALTGSR541VWNNSSTVNA VPVAPPVCDV ARTQPTPSEK AAGVLEGALG PHVVTNLYLY PIKSCAAFEV601TRWPVGNQGL LYDRSWMVVN HNGVCLSQKQ EPRLCLIQPF IDLRQRIMVI KAKGMEPIEV661PLEENSERTQ IRQSRVCADR VSTYDCGEKI SSWLSTFFGR PCHLIKQSSN SQRNAKKKHG721KDQLPGTMAT LSLVNEAQYL LINTSSILEL HRQLNTSDEN GKEELFSLKD LSLRFRANII781INGKRAFEEE KWDEISIGSL RFQVLGPCHR CQMICIDQQT GQRNQHVFQK LSESRETKVN841FGMYLMHASL DLSSPCFLSV GSQVLPVLKE NVEGHDLPAS EKHQDVTSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
888 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.