LSBio
MOB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MOB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108060-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HCCA2(MOB2) (NM_053005), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HCCA2 | MOB kinase activator 2 | MOB2 Mps One Binder homolog | MOB2 | Mob2 homolog
Origin Species
Human
SwissProt
Q70IA6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
26.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDWLMGKSKA KPNGKKPAAE ERKAYLEPEH TKARITDFQF KELVVLPREI DLNEWLASNT
61TTFFHHINLQ YSTISEFCTG ETCQTMAVCN TQYYWYDERG KKVKCTAPQY VDFVMSSVQK
121LVTDEDVFPT KYGREFPSSF ESLVRKICRH LFHVLAHIYW AHFKETLALE LHGHLNTLYV
181HFILFAREFN LLDPKETAIM DDLTEVLCSG AGGVHSGGSG DGAGSGGPGA QNHVKER
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

237 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.