Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
MOAP1 / MAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110426-100
Catalog Number: LS-G110426
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP1(MOAP1) (NM_022151), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAP-1 | MAP1 | Modulator of apoptosis 1 | Paraneoplastic antigen like 4 | Paraneoplastic antigen Ma4 | PNMA4 | MOAP1
- Origin Species
- Human
- NCBI GenBank
- NM_022151
- SwissProt
- Q96BY2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTLRLLEDWC RGMDMNPRKA LLIAGISQSC SVAEIEEALQ AGLAPLGEYR LLGRMFRRDE61NRKVALVGLT AETSHALVPK EIPGKGGIWR VIFKPPDPDN TFLSRLNEFL AGEGMTVGEL121SRALGHENGS LDPEQGMIPE MWAPMLAQAL EALQPALQCL KYKKLRVFSG RESPEPGEEE181FGRWMFHTTQ MIKAWQVPDV EKRRRLLESL RGPALDVIRV LKINNPLITV DECLQALEEV241FGVTDNPREL QVKYLTTYQK DEEKLSAYVL RLEPLLQKLV QRGAIERDAV NQARLDQVIA301GAVHKTIRRE LNLPEDGPAP GFLQLLVLIK DYEAAEEEEA LLQAILEGNF TTRTRPLEQK361LISEEDLAAN DILDYKDDDD KV
382 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.