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MN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

MN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all MN1 antibodies →
$725.00each
Catalog Number: LS-G117988-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MN1 (NM_002430), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ353E16.2 | MGCR1 | MGCR1-PEN | Meningioma chromosome region | Meningioma chromosome region 1 | MGCR | MN1
Origin Species
Human
NCBI GenBank
NM_002430
SwissProt
Q10571

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
135.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFGLDQFEPQ VNSRNAGQGE RNFNETGLSM NTHFKAPAFH TGGPPGPVDP AMSALGEPPI
61LGMNMEPYGF HARGHSELHA GGLQAQPVHG FFGGQQPHHG HPGSHHPHQH HPHFGGNFGG
121PDPGASCLHG GRLLGYGGAA GGLGSQPPFA EGYEHMAESQ GPESFGPQRP GNLPDFHSSG
181ASSHAVPAPC LPLDQSPNRA ASFHGLPSSS GSDSHSLEPR RVTNQGAVDS LEYNYPGEAP
241SGHFDMFSPS DSEGQLPHYA AGRQVPGGAF PGASAMPRAA GMVGLSKMHA QPPQQQPQQQ
301QQPQQQQQQH GVFFERFSGA RKMPVGLEPS VGSRHPLMQP PQQAPPPPQQ QPPQQPPQQQ
361PPPPPGLLVR QNSCPPALPR PQQGEAGTPS GGLQDGGPML PSQHAQFEYP IHRLENRSMH
421PYSEPVFSMQ HPPPQQAPNQ RLQHFDAPPY MNVAKRPRFD FPGSAGVDRC ASWNGSMHNG
481ALDNHLSPSA YPGLPGEFTP PVPDSFPSGP PLQHPAPDHQ SLQQQQQQQQ QQQQQQQQQQ
541QQQQQQQQQQ RQNAALMIKQ MASRNQQQRL RQPNLAQLGH PGDVGQGGLV HGGPVGGLAQ
601PNFEREGGST GAGRLGTFEQ QAPHLAQESA WFSGPHPPPG DLLPRRMGGS GLPADCGPHD
661PSLAPPPPPG GSGVLFRGPL QEPMRMPGEG HVPALPSPGL QFGGSLGGLG QLQSPGAGVG
721LPSAASERRP PPPDFATSAL GGQPGFPFGA AGRQSTPHSG PGVNSPPSAG GGGGSSGGGG
781GGGAYPPQPD FQPSQRTSAS KLGALSLGSF NKPSSKDNLF GQSCLAALST ACQNMIASLG
841APNLNVTFNK KNPPEGKRKL SQNETDGAAV AGNPGSDYFP GGTAPGAPGP GGPSGTSSSG
901SKASGPPNPP AQGDGTSLSP NYTLESTSGN DGKPVSGGGG RGRGRRKRDS GHVSPGTFFD
961KYSAAPDSGG APGVSPGQQQ ASGAAVGGSS AGETRGAPTP HEKALTSPSW GKGAELLLGD
1021QPDLIGSLDG GAKSDSSSPN VGEFASDEVS TSYANEDEVS SSSDNPQALV KASRSPLVTG
1081SPKLPPRGVG AGEHGPKAPP PALGLGIMSN STSTPDSYGG GGGPGHPGTP GLEQVRTPTS
1141SSGAPPPDEI HPLEILQAQI QLQRQQFSIS EDQPLGLKGG KKGECAVGAS GAQNGDSELG
1201SCCSEAVKSA MSTIDLDSLM AEHSAAWYMP ADKALVDSAD DDKTLAPWEK AKPQNPNSKE
1261AHDLPANKAS ASQPGSHLQC LSVHCTDDVG DAKARASVPT WRSLHSDISN RFGTFVAALT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1320 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.